巴西朗多尼亚慢性 HCV 感染者 HCV 基因型分布。

Distribution of hepatitis c virus (hcv) genotypes in patients with chronic infection from Rondônia, Brazil.

机构信息

Laboratory of Tropical Gastroenterology and Hepatology, São Paulo Institute of Tropical Medicine and Department of Gastroenterology, School of Medicine, University of São Paulo, São Paulo SP, Brazil.

出版信息

Virol J. 2011 Apr 12;8:165. doi: 10.1186/1743-422X-8-165.

DOI:10.1186/1743-422X-8-165

PMID:21486472

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3082223/

Abstract

BACKGROUND

Hepatitis C virus (HCV) is an important human pathogen affecting around 3% of the human population. In Brazil, it is estimated that there are approximately 2 to 3 million HCV chronic carriers. There are few reports of HCV prevalence in Rondônia State (RO), but it was estimated in 9.7% from 1999 to 2005. The aim of this study was to characterize HCV genotypes in 58 chronic HCV infected patients from Porto Velho, Rondônia (RO), Brazil.

METHODS

A fragment of 380 bp of NS5B region was amplified by nested PCR for genotyping analysis. Viral sequences were characterized by phylogenetic analysis using reference sequences obtained from the GenBank (n = 173). Sequences were aligned using Muscle software and edited in the SE-AL software. Phylogenetic analyses were conducted using Bayesian Markov chain Monte Carlo simulation (MCMC) to obtain the MCC tree using BEAST v.1.5.3.

RESULTS

From 58 anti-HCV positive samples, 22 were positive to the NS5B fragment and successfully sequenced. Genotype 1b was the most prevalent in this population (50%), followed by 1a (27.2%), 2b (13.6%) and 3a (9.0%).

CONCLUSIONS

This study is the first report of HCV genotypes from Rondônia State and subtype 1b was found to be the most prevalent. This subtype is mostly found among people who have a previous history of blood transfusion but more detailed studies with a larger number of patients are necessary to understand the HCV dynamics in the population of Rondônia State, Brazil.

摘要

背景

丙型肝炎病毒（HCV）是一种重要的人类病原体，影响全球约 3%的人口。在巴西，估计约有 200 万至 300 万 HCV 慢性携带者。关于朗多尼亚州（RO）的 HCV 流行率的报告较少，但据估计，1999 年至 2005 年期间为 9.7%。本研究旨在对来自巴西朗多尼亚州（RO）波多韦柳市的 58 名慢性 HCV 感染患者的 HCV 基因型进行特征描述。

方法

采用巢式 PCR 扩增 NS5B 区 380bp 片段进行基因分型分析。使用从 GenBank 获得的参考序列（n=173）通过系统发育分析对病毒序列进行特征描述。使用 Muscle 软件进行序列比对，并在 SE-AL 软件中进行编辑。使用贝叶斯马尔可夫链蒙特卡罗模拟（MCMC）通过 BEAST v.1.5.3 进行系统发育分析，以获得 MCC 树。