School of Food Technology and Nutrition, Chonnam National University, Yeosu 550-749, Republic of Korea.
Bioorg Med Chem Lett. 2011 May 15;21(10):3070-3. doi: 10.1016/j.bmcl.2011.03.033. Epub 2011 Mar 16.
Chitosan gallate were synthesized using a free radical-induced grafting reaction. Chitosan gallate showed enhanced water-solubility compared to plain chitosan, and exhibited good thermal stability. The IC(50) value of chitosan gallate against 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 17.86 μg/mL. In addition, chitosan gallate effectively inhibited the generation of intracellular reactive oxygen species (ROS), and also suppressed lipid peroxidation in RAW264.7 macrophage cells. Chitosan gallate also exhibited the protection effect on genomic DNA damage by induced hydroxyl radical, and up-regulated the protein expression of antioxidant enzymes including superoxide dismutase-1 and glutathione reductase under H(2)O(2)-mediated oxidative stress in RAW264.7 macrophage cells. These results indicate that chitosan gallate might be potential antioxidant biomaterials.
没食子酸壳聚糖是通过自由基诱导接枝反应合成的。与壳聚糖相比，没食子酸壳聚糖的水溶性得到了提高，同时表现出良好的热稳定性。没食子酸壳聚糖对 2,2-二苯基-1-苦肼基（DPPH）的 IC50 值为 17.86μg/mL。此外，没食子酸壳聚糖能有效抑制 RAW264.7 巨噬细胞内活性氧（ROS）的产生，抑制脂质过氧化。没食子酸壳聚糖还能对羟自由基诱导的基因组 DNA 损伤起到保护作用，并在 RAW264.7 巨噬细胞中上调超氧化物歧化酶-1 和谷胱甘肽还原酶等抗氧化酶的蛋白表达，在 H2O2 介导的氧化应激下。这些结果表明，没食子酸壳聚糖可能是一种有潜力的抗氧化生物材料。
