School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
J Agric Food Chem. 2013 Jul 17;61(28):6921-8. doi: 10.1021/jf402242e. Epub 2013 Jul 8.
The objective of this study was to evaluate the in vitro antioxidant activity of high molecular weight chitosan based films. Three kinds of water-soluble quaternized chitosans with high molecular weight, namely N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (400-HTCC and 1240-HTCC), N-(2-hydroxyl) propyl-3-triethyl ammonium chitosan chloride (400-HTEC and 1240-HTEC), and O-(2-hydroxyl) propyl-3- trimethyl ammonium chitosan chloride (400-O-HTCC) were prepared from high molecular weight chitosans (400 and 1240 kDa). The in vitro antioxidant activity of a high molecular weight chitosan (1240-CS) and five quaternized chitosans was evaluated and compared as radical scavengers against 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH•), hydroxyl radical (•OH), and superoxide radical (•O2(-)) using established methods, and the effect of the molecular weight, the concentration, the newly generated hydroxyl group, the extra introduced positive charge of quaternary ammonium salt group, etc., on the antioxidant activity of these high molecular weight chitosans is discussed. The data obtained in vitro models exhibited good antioxidant potency and suggested the possibility that high molecular weight chitosan based films could be effectively employed as natural antioxidant materials for application in the field of food and medicine.
本研究旨在评估基于高分子量壳聚糖的薄膜的体外抗氧化活性。三种水溶性季铵化壳聚糖，即 N-(2-羟基)丙基-3-三甲基氯化铵壳聚糖（400-HTCC 和 1240-HTCC）、N-(2-羟基)丙基-3-三乙基氯化铵壳聚糖（400-HTEC 和 1240-HTEC）和 O-(2-羟基)丙基-3-三甲基氯化铵壳聚糖（400-O-HTCC），是由高分子量壳聚糖（400 和 1240 kDa）制备的。使用已建立的方法，作为自由基清除剂，评估和比较了高分子量壳聚糖（1240-CS）和五种季铵化壳聚糖对 1,1-二苯基-2-苦基肼自由基（DPPH•）、羟基自由基（•OH）和超氧自由基（•O2(-)）的体外抗氧化活性，并讨论了分子量、浓度、新生成的羟基、季铵盐基团引入的额外正电荷等对这些高分子量壳聚糖抗氧化活性的影响。体外模型中获得的数据显示出良好的抗氧化能力，这表明基于高分子量壳聚糖的薄膜可能作为天然抗氧化材料有效应用于食品和医药领域。
