4th Department of Internal Medicine, Athens University School of Medicine, University General Hospital ATTIKON, Chaidari, Greece.
Int J Antimicrob Agents. 2011 Feb;37(2):124-8. doi: 10.1016/j.ijantimicag.2010.10.010. Epub 2010 Dec 21.
In this study, the performance of the chromogenic medium CHROMagar™ KPC was evaluated and was compared with in-house-daily prepared McConkey agar plates supplemented with imipenem (1 mg/L) for the detection of carbapenemase-producing Enterobacteriaceae. In this surveillance study, rectal swabs were cultured on both media and polymerase chain reaction (PCR) for bla(KPC) and bla(VIM) was used to confirm the genotype of growing colonies of Enterobacteriaceae. CHROMagar KPC was also tested with 17 genotypically characterised carbapenemase-producing and non-producing Gram-negative bacteria. It was shown that CHROMagar allows rapid detection of carbapenemase-producing Enterobacteriaceae, although bla(KPC)- and bla(VIM)-harbouring isolates could not be differentiated by colour or colony morphology. The positive and negative predictive values of the tested methods for the detection of carbapenemase-producing Enterobacteriaceae were, respectively, 100% and 98.8% for CHROMagar KPC and 94.7% and 88.6% for imipenem-supplemented McConkey agar. CHROMagar KPC medium is a useful screening medium for carbapenemase-producing Enterobacteriaceae in stools in settings with a high proportion of patients colonised with a variety of carbapenemase-producers.
在这项研究中，评估了显色培养基 CHROMagar™ KPC 的性能，并将其与自制的补充亚胺培南（1mg/L）的麦康凯琼脂平板进行比较，用于检测产碳青霉烯酶的肠杆菌科。在这项监测研究中，直肠拭子在两种培养基上培养，并使用聚合酶链反应（PCR）检测 bla(KPC)和 bla(VIM)，以确认肠杆菌科生长菌落的基因型。CHROMagar KPC 还与 17 种经过基因分型的产碳青霉烯酶和非产碳青霉烯酶的革兰氏阴性菌进行了测试。结果表明，CHROMagar 允许快速检测产碳青霉烯酶的肠杆菌科，尽管 bla(KPC)和 bla(VIM)携带的分离株不能通过颜色或菌落形态来区分。对于检测产碳青霉烯酶的肠杆菌科，所测试方法的阳性和阴性预测值分别为 CHROMagar KPC 的 100%和 98.8%，亚胺培南补充麦康凯琼脂的 94.7%和 88.6%。CHROMagar KPC 培养基是一种在患者定植多种产碳青霉烯酶菌比例较高的情况下，用于粪便中产碳青霉烯酶肠杆菌科的有用筛选培养基。
