Department of Gastroenterology, Ankara Education and Research Hospital, Ankara, Turkey.
Clin Res Hepatol Gastroenterol. 2011 Sep;35(8-9):549-53. doi: 10.1016/j.clinre.2011.02.013. Epub 2011 Apr 12.
Percutaneous ethanol injection is an established management of nonresectable hepatocellular carcinoma (HCC) because of its high effectiveness and minimal invasiveness. However, ethanol has many disadvantages like less anti-tumoral necrotic effectivity, unequal permeation and local diffusion. The aim of this study is to compare hepatic tissue effects of percutaneous Ankaferd Blood Stopper (ABS) injection in comparison to ethanol in rat liver tissue.
Twenty-one healthy rats were randomly divided into three groups, each containing seven animals. Group I received 0.1cm(3) percutaneous injection of isotonic saline, group II received 0.1cm(3) ethanol, and group III received 0.1cm(3) ABS. At the 5th day, the livers were dissected. Macroscopic and histopathological features of the liver lesions were documented.
All the rats in the group I and II lived during study period; one rat died in the ABS group. Macroscopic pale yellow coloration was observed within 2 minutes in both ethanol and ABS groups. Necrosis was observed in both Group II and III. The necrosis volumes of the ABS group (volume: 1475.00 ± 697.16 cm(3)) were significantly higher than the ethanol group (volume: 60.714 ± 26.277 cm(3)) (P=0.002). In the histopathological analyses of the liver tissues, aggregated erythrocytes in sinusoidal spaces and bile duct proliferation have been detected in ABS group.
ABS may be considered as a possible percutaneous treatment in HCC instead of or as an alternative to ethanol. With its unique hemostatic actions and the safety profile, ABS can be considered as a useful novel agent for the percutaneous therapy of HCC instead of ethanol in the future.
经皮乙醇注射是一种已确立的不可切除肝细胞癌（HCC）的治疗方法，因为它具有高效性和最小的侵入性。然而，乙醇有许多缺点，如抗肿瘤坏死效果较差、渗透和局部扩散不均匀。本研究的目的是比较经皮安卡福止血海绵（ABS）注射与乙醇在大鼠肝组织中的肝组织效应。
21 只健康大鼠随机分为三组，每组 7 只。第 I 组接受 0.1cm³经皮注射等渗盐水，第 II 组接受 0.1cm³乙醇，第 III 组接受 0.1cm³ ABS。第 5 天，解剖肝脏。记录肝脏病变的大体和组织病理学特征。
第 I 组和第 II 组的所有大鼠在研究期间均存活；ABS 组有一只大鼠死亡。乙醇和 ABS 组在 2 分钟内均观察到肝组织呈浅黄色。第 II 组和第 III 组均观察到坏死。ABS 组的坏死体积（体积：1475.00 ± 697.16 cm³）明显高于乙醇组（体积：60.714 ± 26.277 cm³）（P=0.002）。在肝组织的组织病理学分析中，ABS 组观察到窦状隙中聚集的红细胞和胆管增殖。
ABS 可被认为是 HCC 的一种可行的经皮治疗方法，可替代或作为乙醇的替代品。由于其独特的止血作用和安全性，ABS 可被认为是未来 HCC 经皮治疗的一种有用的新型药物，替代乙醇。
