Alpay Atilla, Bektas Sibel, Alpay Aysegul, Ugurbas Silay Canturk, Evren Cenk, Ugurbas Suat Hayri
Department of Ophthalmology, Zonguldak Karaelmas University, Göz Hastaliklari Anabilim Dali, Kozlu 67600, Zonguldak, Turkey.
Cutan Ocul Toxicol. 2012 Jun;31(2):128-31. doi: 10.3109/15569527.2011.627577. Epub 2011 Oct 28.
To investigate the histopathological changes due to administration of Ankaferd Blood Stopper(®) (ABS) into intraocular tissues by an anterior chamber and intravitreal injections.
Twenty Wistar albino rats were divided into four equal groups. Group 1 was injected 0.01 mL ABS into anterior chamber. Group 2 was injected intravitreal 0.02 mL ABS. Groups 3 and 4, which were used as controls, were injected into the anterior chamber and intravitreal 0.01 mL and 0.02 mL balanced salt solution (BSS), respectively. At 2, 5, 10, 15 and 20 days after injection, the eyes were examined under an operating microscope and were subsequently enucleated for histopathological examination.
Ophthalmic examination of the rats prior to enucleation revealed ocular complications ranging from conjunctival hyperemia to corneal perforation in group 1 and increased conjunctival hyperemia and discharge in group 2. No physical and histopathological anomalies were detected in groups 3 and 4. All eyes in group 1 showed mixed type inflammatory cell reaction, foreign-body reaction, stromal congestion, disintegration of the collagen fibers and loss of the epithelium of the posterior wall in the iris and ciliary body were observed histopathologically. All eyes in group 2 showed disintegration and separation of the retina, brown pigment accumulation and mixed type inflammatory cell reaction.
Our results indicate that the commercially available form of ABS solution exerts a toxic effect on intraocular tissues. We consider that the intraocular use of different concentrations, rather than multiple time point of ABS should be investigated.
通过前房注射和玻璃体腔注射，研究安卡芬德止血剂（Ankaferd Blood Stopper，®）（ABS）注入眼内组织后的组织病理学变化。
将20只Wistar白化大鼠平均分为四组。第1组向前房注射0.01 mL ABS。第2组向玻璃体腔注射0.02 mL ABS。作为对照组的第3组和第4组，分别向前房和玻璃体腔注射0.01 mL和0.02 mL平衡盐溶液（BSS）。在注射后2、5、10、15和20天，在手术显微镜下检查眼睛，随后摘除眼球进行组织病理学检查。
摘除眼球前对大鼠的眼科检查显示，第1组出现从结膜充血到角膜穿孔的眼部并发症，第2组结膜充血和分泌物增多。第3组和第4组未检测到物理和组织病理学异常。组织病理学观察发现，第1组所有眼睛均出现混合型炎症细胞反应、异物反应、基质充血、虹膜和睫状体胶原纤维崩解以及后壁上皮缺失。第2组所有眼睛均出现视网膜崩解和分离、棕色色素沉着以及混合型炎症细胞反应。
我们的结果表明，市售形式的ABS溶液对眼内组织有毒性作用。我们认为应研究不同浓度而非多个时间点的ABS在眼内的使用情况。
