Department of Veterinary Infectious Diseases, Chonbuk National University, Jeonju, Korea.
Poult Sci. 2011 May;90(5):977-82. doi: 10.3382/ps.2010-01160.
Proanthocyanidins are naturally occurring compounds that are widely found in fruits, vegetables, nuts, seeds, flowers, and bark. We evaluated the immunomodulatory effects of proanthocyanidin-rich extract (PAE) from Pinus radiata bark in specific-pathogen-free White Leghorn chickens. Proliferation of peripheral blood mononuclear cells was significantly enhanced in chickens treated for 2 wk with 20 mg/kg of PAE. Proliferation of splenocytes and bursal cells was significantly enhanced in chickens treated for 5 wk with 5, 10, and 20 mg/kg of PAE. Thymocyte proliferation was significantly enhanced in chickens treated for 5 wk with 5 and 10 mg/kg of PAE. These effects were markedly enhanced by the presence of lipopolysaccharide, which acted on B cells responsible for humoral immunity, and concanavalin A, which acted directly on T cells involved in cell-mediated immunity. The PAE significantly promoted the expression of T helper 1 cytokine (interferon-γ) and decreased the expression of T helper 2 cytokine (IL-6). Thus, P. radiata PAE has immunomodulatory effects in specific-pathogen-free White Leghorn chickens.
原花青素是广泛存在于水果、蔬菜、坚果、种子、花和树皮中的天然化合物。我们评估了来自辐射松树皮的原花青素丰富提取物（PAE）对无特定病原体白来航鸡的免疫调节作用。用 20mg/kg 的 PAE 处理 2 周的鸡的外周血单核细胞增殖显著增强。用 5、10 和 20mg/kg 的 PAE 处理 5 周的鸡的脾细胞和腔上囊细胞增殖显著增强。用 5 和 10mg/kg 的 PAE 处理 5 周的鸡的胸腺细胞增殖显著增强。脂多糖存在时，这些作用明显增强，脂多糖作用于负责体液免疫的 B 细胞，刀豆蛋白 A 直接作用于参与细胞介导免疫的 T 细胞。PAE 显著促进了辅助性 T 细胞 1 型细胞因子（干扰素-γ）的表达，降低了辅助性 T 细胞 2 型细胞因子（IL-6）的表达。因此，辐射松 PAE 对无特定病原体白来航鸡具有免疫调节作用。
