Department of Biotechnology, Akita Prefectural University, Akita City, Akita 010-0195 Japan.
Poult Sci. 2011 May;90(5):1004-8. doi: 10.3382/ps.2010-01153.
The transition of the bacterial community structure and predominant bacteria in the ceca of chicks from hatching to 2 wk of age was investigated using denaturing gradient gel electrophoresis with the 16S ribosomal RNA gene, followed by phylogenetic analysis. The results demonstrated that most of the cecal bacterial flora from hatching to a few days old consisted of Escherichia coli (sequence similarity: 100%), and the floral diversity was still low 2 wk posthatch. These findings will help contribute to the development of a novel competitive exclusion product.
采用变性梯度凝胶电泳和 16S 核糖体 RNA 基因测序技术,并结合系统发育分析方法,研究了从出雏到 2 周龄雏鸡盲肠细菌群落结构及其优势菌的变化。结果表明,出雏后至雏鸡 1 日龄盲肠内容物的细菌菌群主要为大肠杆菌(序列相似性为 100%),出雏后 2 周内菌群多样性仍然较低。这些发现将有助于开发新型竞争性排斥产品。