Department of Animal Science, University of California, Davis, CA, 95616, USA.
State Key Laboratory of Animal Science, China Agricultural University, Beijing, China.
Sci Rep. 2020 Mar 16;10(1):4809. doi: 10.1038/s41598-020-60892-9.
Intestinal carriage of Salmonella Enteritidis (SE) in the chicken host serves as a reservoir for transmission of Salmonella to humans through the consumption of poultry products. The aim of the current study was to examine the three-way interaction that occurred between host metabolites, resident gut microbiota and Salmonella following inoculation of SE in two-week-old layer chicks. Our results revealed an overall alteration in gut microbiome and metabolites in association with SE infection. Enriched colonization by different microbial members throughout the course of experimental infection highlighted significant fluctuation in the intestinal microbial community in response to Salmonella infection. As changes in community membership occurred, there was also subsequent impact on differential regulation of interlinked predicted functional activities within the intestinal environment dictated by Salmonella-commensal interaction. Alteration in the overall microbial community following infection also has a ripple effect on the host regulation of cecum-associated metabolic networks. The findings showed that there was differential regulation in many of the metabolites in association with SE colonization in chickens. Perturbation in metabolic pathways related to arginine and proline metabolism as well as TCA cycle was most prominently detected. Taken together, the present findings provided a starting point in understanding the effect of intestinal Salmonella carriage on the microbiome and metabolome of developing young layer chicks.
肠道中肠炎沙门氏菌（SE）的携带是通过食用家禽产品将沙门氏菌传播给人类的宿主储层。本研究的目的是研究在两周大的蛋鸡中接种 SE 后，宿主代谢物、常驻肠道微生物群和沙门氏菌之间发生的三向相互作用。我们的结果显示，肠道微生物群和代谢物的整体变化与 SE 感染有关。在整个实验感染过程中，不同微生物成员的富集定植突出表明，肠道微生物群落对沙门氏菌感染的反应存在显著波动。随着群落成员的变化，沙门氏菌-共生体相互作用所决定的肠道环境中相互关联的预测功能活性的差异调节也随之受到影响。感染后整个微生物群落的改变也对宿主对盲肠相关代谢网络的调节产生连锁反应。研究结果表明，在与鸡 SE 定植相关的许多代谢物中存在差异调节。与精氨酸和脯氨酸代谢以及 TCA 循环相关的代谢途径的扰动最为明显。总之，本研究结果为了解肠道沙门氏菌携带对发育中年轻蛋鸡微生物组和代谢组的影响提供了一个起点。
