Biotechnology & Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg, Germany.
Molecules. 2011 Apr 13;16(4):3106-18. doi: 10.3390/molecules16043106.
We review fluorescent probes that can be photoswitched or photoactivated and are suited for single-molecule localization based super-resolution microscopy. We exploit the underlying photochemical mechanisms that allow photoswitching of many synthetic organic fluorophores in the presence of reducing agents, and study the impact of these on the photoswitching properties of various photoactivatable or photoconvertible fluorescent proteins. We have identified mEos2 as a fluorescent protein that exhibits reversible photoswitching under various imaging buffer conditions and present strategies to characterize reversible photoswitching. Finally, we discuss opportunities to combine fluorescent proteins with organic fluorophores for dual-color photoswitching microscopy.
我们综述了可光开关或光激活的荧光探针，这些探针适用于基于单分子定位的超分辨率显微镜。我们利用允许在还原剂存在下对许多合成有机荧光团进行光开关的基本光化学机制，并研究这些对各种光活化或光转化荧光蛋白的光开关性质的影响。我们已经确定 mEos2 是一种荧光蛋白，它在各种成像缓冲条件下表现出可逆的光开关，并提出了用于表征可逆光开关的策略。最后，我们讨论了将荧光蛋白与有机荧光团结合用于双色光开关显微镜的机会。
