Nanguneri Siddharth, Flottmann Benjamin, Herrmannsdörfer Frank, Thomas Kuner, Heilemann Mike
Institute of Anatomy and Cell Biology, Heidelberg University, INF 307, 69120, Heidelberg, Germany.
Microsc Res Tech. 2014 Jul;77(7):510-6. doi: 10.1002/jemt.22349. Epub 2014 Mar 5.
Photophysical properties of any fluorophore are governed by the chemical nanoenvironment. In the context of imaging biological samples, this translates to different photophysical properties for different labels and probes. Here, we demonstrate that the nanoenvironment of fluorophores within a probe can be advantageously used to induce particular properties such as light-induced photoswitching. We demonstrate efficient photoswitching and single-molecule super-resolution imaging for various fluorophore-phalloidin conjugates in aqueous buffer without the addition of further chemicals. We further demonstrate the utility of two-color imaging of fluorophore-phalloidin and a photoactivatable fluorescent protein in presynaptic nerve terminals.
任何荧光团的光物理性质都受化学纳米环境的支配。在对生物样品进行成像的背景下，这意味着不同的标记物和探针具有不同的光物理性质。在此，我们证明，探针内荧光团的纳米环境可被有效地用于诱导特定性质，如光诱导光开关。我们展示了在不添加其他化学物质的情况下，各种荧光团-鬼笔环肽缀合物在水性缓冲液中的高效光开关和单分子超分辨率成像。我们还展示了荧光团-鬼笔环肽与光活化荧光蛋白在突触前神经末梢进行双色成像的实用性。
