Department of Biological Information, Tokyo Institute of Technology, Yokohama, Japan.
PLoS One. 2011 Apr 7;6(4):e18410. doi: 10.1371/journal.pone.0018410.
Matricellular proteins, including periostin, are important for tissue regeneration.
Presently we investigated the function of periostin in cutaneous wound healing by using periostin-deficient ⁻/⁻ mice. Periostin mRNA was expressed in both the epidermis and hair follicles, and periostin protein was located at the basement membrane in the hair follicles together with fibronectin and laminin γ2. Periostin was associated with laminin γ2, and this association enhanced the proteolytic cleavage of the laminin γ2 long form to produce its short form. To address the role of periostin in wound healing, we employed a wound healing model using WT and periostin⁻/⁻ mice and the scratch wound assay in vitro. We found that the wound closure was delayed in the periostin⁻/⁻ mice coupled with a delay in re-epithelialization and with reduced proliferation of keratinocytes. Furthermore, keratinocyte proliferation was enhanced in periostin-overexpressing HaCaT cells along with up-regulation of phosphorylated NF-κB.
These results indicate that periostin was essential for keratinocyte proliferation for re-epithelialization during cutaneous wound healing.
细胞外基质蛋白，包括骨膜蛋白，对于组织再生非常重要。
目前，我们通过使用骨膜蛋白缺陷 ⁻/⁻ 小鼠来研究骨膜蛋白在皮肤伤口愈合中的功能。骨膜蛋白 mRNA 在表皮和毛囊中均有表达，骨膜蛋白蛋白与纤连蛋白和层粘连蛋白 γ2 一起位于毛囊的基底膜上。骨膜蛋白与层粘连蛋白 γ2 相关联，这种关联增强了层粘连蛋白 γ2 长形式的蛋白水解裂解，从而产生其短形式。为了研究骨膜蛋白在伤口愈合中的作用，我们使用 WT 和骨膜蛋白 ⁻/⁻ 小鼠以及体外划痕实验建立了伤口愈合模型。我们发现骨膜蛋白 ⁻/⁻ 小鼠的伤口闭合延迟，伴随着上皮再形成的延迟和角质形成细胞增殖减少。此外，骨膜蛋白过表达的 HaCaT 细胞中的角质形成细胞增殖增强，同时 NF-κB 的磷酸化水平上调。
这些结果表明，骨膜蛋白对于皮肤伤口愈合过程中的角质形成细胞增殖和上皮再形成至关重要。
