Department of Environmental Biology and Chemistry, Graduate School of Science and Engineering for Research, University of Toyama, Gofuku 3190, Toyama, 930-8555, Japan.
Analyst. 2011 Jun 7;136(11):2373-8. doi: 10.1039/c1an15100b. Epub 2011 Apr 14.
A simple electrochemical binding assay for cholera toxin (CT) was developed using lactose labeled with daunomycin as an electroactive compound. The labeled lactose (LL) was determined with high sensitivity by adsorptive stripping voltammetry (AdSV). The electrochemical behaviors of LL at glassy carbon (GC), plastic formed carbon (PFC) and carbon nanotubes paste (CNTP) electrode were investigated. The CNTP electrode showed the greatest accumulation capacity for LL. The assay for CT based on the sequestration electrochemistry was demonstrated. The binding event of the LL to CT was detected by the decrease in the electrochemical response of daunomycin as an electroactive label without a separation process to remove the free LL from the one bound with CT before any measurements can be made. The detection limit of the CT assay using the CNTP electrode was 0.5 nM (42 ng mL(-1)).
采用道诺霉素标记的乳糖作为电化学活性化合物，建立了一种简单的霍乱毒素（CT）电化学结合分析方法。采用示差脉冲伏安法（AdSV）高灵敏度测定标记乳糖（LL）。研究了 LL 在玻碳电极（GC）、塑料成型碳电极（PFC）和碳纳米管糊电极（CNTP）上的电化学行为。CNTP 电极对 LL 具有最大的富集能力。基于螯合电化学的 CT 检测得到了证明。通过将 LL 与 CT 的结合物从游离的 LL 中分离出来，检测到 LL 与 CT 的结合事件，从而减少了作为电化学标记的道诺霉素的电化学响应，而无需进行分离过程。使用 CNTP 电极检测 CT 的检测限为 0.5 nM（42 ng mL（-1））。
