Department of Nanoengineering, University of California-San Diego, La Jolla, California 92093, United States.
Nano Lett. 2011 May 11;11(5):2083-7. doi: 10.1021/nl2005687. Epub 2011 Apr 14.
The transport properties of single-strand DNA probe-modified self-propelling micromachines are exploited for "on-the-fly" hybridization and selective single-step isolation of target nucleic acids from "raw" microliter biological samples (serum, urine, crude E. coli lysate, saliva). The rapid movement of the guided modified microrockets induces fluid convection, which enhances the hybridization efficiency, thus enabling the rapid and selective isolation of nucleic acid targets from untreated samples. The integration of these autonomous microrockets into a lab-on-chip device that provides both nucleic acid isolation and downstream analysis could thus be attractive for diverse applications.
单链 DNA 探针修饰的自推进微型机器的传输特性被用于“实时”杂交，并从“原始”微升生物样品（血清、尿液、粗大肠杆菌裂解液、唾液）中选择性地一步分离靶核酸。受引导的修饰微型火箭的快速运动引起流体对流，从而提高杂交效率，从而能够从未处理的样品中快速且选择性地分离核酸靶标。将这些自主微型火箭集成到提供核酸分离和下游分析的芯片实验室设备中，对于各种应用可能具有吸引力。
