State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, China.
Can J Microbiol. 2011 Apr;57(4):263-72. doi: 10.1139/w11-003.
Bacterial community structure and the effects of several environmental factors on bacterial community distribution were investigated in the sediment of the macrophyte-dominated and algae-dominated areas in a large, shallow, eutrophic freshwater lake (Lake Taihu, China). Surface sediment samples were collected at 6 sampling sites (3 sites from each of the 2 areas) on 15 February and 15 August 2009. Based on cluster analysis of the DGGE banding patterns, there were significant seasonal variations in the structure of the sediment bacterial community in the macrophyte- and algae-dominated areas, and site-specific variation within an area and between 2 areas. However, there were no significant between-area variations due to the large within-area variation. Analysis of DNA sequences showed that there were differences in the species composition of the sediment bacteria between the macrophyte- and algae-dominated area clone libraries. In the macrophyte-dominated area library, the bacterial community was dominated by Deltaproteobacteria, Verrucomicrobia, Acidobacteria, Bacteroidetes, Gammaproteobacteria, and Betaproteobacteria. OP10 was found in the library of this area but not in the algae-dominated area library. The algae-dominated area library was dominated by Betaproteobacteria, Deltaproteobacteria, Gammaproteobacteria, and Acidobacteria. Cyanobacteria, Alphaproteobacteria, and Planctomycetes were found in this area library but not in the macrophyte-dominated area library. Canonical correspondence analysis demonstrated that total phosphorus and water temperature were the dominant environmental factors affecting bacterial community composition in the sediment.
采用 DGGE 指纹图谱技术分析了大型浅水富营养化湖泊（中国太湖）沉水植物区和藻类区沉积物中细菌群落结构及其分布特征，并探讨了几种环境因子对细菌群落分布的影响。于 2009 年 2 月 15 日和 8 月 15 日在各分区的 3 个采样点采集表层沉积物样品。聚类分析结果表明，沉水植物区和藻类区沉积物细菌群落结构具有明显的季节性变化，且同一分区内不同采样点之间和不同分区之间也存在着特异性变化，但由于分区内变化较大，不同分区之间并无显著差异。DNA 序列分析结果表明，沉水植物区和藻类区沉积物细菌的种属组成存在差异。在沉水植物区，优势菌门为变形菌门（Deltaproteobacteria）、疣微菌门（Verrucomicrobia）、酸杆菌门（Acidobacteria）、拟杆菌门（Bacteroidetes）、γ-变形菌纲（Gammaproteobacteria）和β-变形菌纲（Betaproteobacteria），且 OP10 仅存在于沉水植物区克隆文库中。藻类区的优势菌门为β-变形菌纲（Betaproteobacteria）、δ-变形菌纲（Deltaproteobacteria）、γ-变形菌纲（Gammaproteobacteria）和酸杆菌门（Acidobacteria），且蓝藻门（Cyanobacteria）、α-变形菌纲（Alphaproteobacteria）和浮霉菌门（Planctomycetes）仅存在于藻类区克隆文库中。典范对应分析结果表明，总磷和水温是影响沉积物细菌群落结构的主要环境因子。
