荧光剧院：一种使用戏剧凝胶进行荧光蛋白和染料筛选的具有成本效益的设备。
The fluorescence theatre: a cost-effective device using theatre gels for fluorescent protein and dye screening.
机构信息
Department of Biology, University of Waterloo, ON, Canada.
出版信息
Can J Microbiol. 2011 Apr;57(4):339-42. doi: 10.1139/w11-010.
DOI:10.1139/w11-010
PMID:21491986
Abstract
Here we report a simple cost-effective device for screening colonies on plates for expression of the monomeric red fluorescent protein mRFP1 and the fluorescent dye Nile red. This device can be built from any simple light source, in our case a Quebec Colony Counter, and cost-effective theatre gels. The device can be assembled in as little as 20 min, and it produces excellent results when screening a large number of colonies.
摘要
在这里，我们报告了一种简单且经济有效的设备，用于筛选平板上表达单体红色荧光蛋白 mRFP1 和荧光染料尼罗红的菌落。该设备可以由任何简单的光源构建，在我们的情况下是一个魁北克菌落计数器，以及经济实惠的戏剧凝胶。该设备可以在短短 20 分钟内组装完成，并且在筛选大量菌落时可以产生出色的结果。
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