Department of Food Science and Technology, UAM Reynosa Aztlán, University Autónoma de Tamaulipas , Reynosa, Tamaulipas, Mexico.
Foodborne Pathog Dis. 2011 Jul;8(7):815-23. doi: 10.1089/fpd.2010.0811. Epub 2011 Apr 14.
Mexican fruit fly Anastrepha ludens Loew (Diptera: Tephritidae) is one of the most important insects infesting fruits. Although high pressure has been proposed as an alternative quarantine process for this pest, conditions applied to destroy eggs and larvae can also damage the fruits. The objective of this study was to assess the biological viability of A. ludens eggs treated by high-pressure processing at 0°C, establishing whether nondestroyed eggs and larvae preserved their ability to develop and reproduce. One-, 2-, 3-, and 4-day-old eggs were pressurized at 50, 70, or 90 MPa for 0, 3, 6, or 9 min. The hatching ability of pressurized eggs and their capacity to develop larvae, pupae, and reproductive adults were evaluated. The ability of pressurized larvae to pupate was also registered. The results showed that most of the eggs that resisted the treatments were able to produce adults with capability to reproduce a new generation of A. ludens flies. Larvae were less resistant than eggs. All larvae were killed at 90 MPa for 9 min. The pressure induced the expression of heat shock proteins (HSP) in second- and third-instar larvae. The HSP showed a baroprotective effect in A. ludens larvae. These results are relevant to the industry because they show that eggs of insects infesting fruits treated by high-pressure processing were able to pupate after treatments. This indicates that efforts must be addressed to destroy all eggs because most of the surviving organisms could reach an adult stage and reproduce.
墨西哥实蝇 Anastrepha ludens Loew（双翅目：实蝇科）是危害水果的最重要昆虫之一。尽管高压已被提议作为该害虫的替代检疫处理方法，但用于破坏卵和幼虫的条件也会损坏水果。本研究的目的是评估在 0°C 下用高压处理的 A. ludens 卵的生物生存能力，确定未被破坏的卵和幼虫是否保持了发育和繁殖的能力。将 1、2、3 和 4 天龄的卵在 50、70 或 90 MPa 下加压 0、3、6 或 9 分钟。评估了加压卵的孵化能力及其发育成幼虫、蛹和有生殖能力的成虫的能力。还记录了加压幼虫化蛹的能力。结果表明，大多数抵抗处理的卵能够产生具有繁殖新一代 A. ludens 蝇能力的成虫。幼虫比卵更具抵抗力。所有幼虫在 90 MPa 下加压 9 分钟都会死亡。压力诱导了第二和第三龄幼虫中热休克蛋白 (HSP) 的表达。HSP 在 A. ludens 幼虫中表现出了一种抗压力的作用。这些结果与工业相关，因为它们表明经高压处理的水果害虫卵在处理后能够化蛹。这表明必须努力消灭所有的卵，因为大多数存活的生物都可能达到成虫阶段并繁殖。
