Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Provincial Key Laboratory of Biotechnology, Institute of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, People's Republic of China.
J Plant Physiol. 2011 Sep 1;168(13):1576-87. doi: 10.1016/j.jplph.2011.01.023. Epub 2011 Apr 14.
AZI1 (AZELAIC ACID INDUCED 1) of Arabidopsis thaliana could be induced by azelaic acid and was involved in priming of systemic plant immunity. In the present work, expression of AZI1 in response to low temperature was investigated via RNA gel blot analysis. AZI1 could be induced slowly by cold stress and more than 6h treatment at 4°C was required to detect an increase in mRNA abundance. However, the high expression state could not be maintained stably and would decline to basal level when the plants were transferred to room temperature. In order to clarify the function of AZI1 in resistance to abiotic stresses, overexpressing, RNA interference and T-DNA knockout lines of this gene were used in electrolyte leakage assays. Overexpression of AZI1 resulted in reduced electrolyte leakage during freezing damage. In contrast, AZI1 knockdown and knockout lines showed increased tendencies in cellular damage after freezing treatment. To further validate the potential resistance of AZI1 to low-temperature stress, Saccharomyces cerevisiae cells were transformed with pESC-AZI1 in which AZI1 was under the control of GAL1 promoter. Compared to yeast cells containing empty pESC-URA, the survival rate of yeast cells harboring AZI1 increased obviously after freezing treatment. All these results suggested that AZI1 might be multifunctional and associated with cold tolerance of Arabidopsis.
拟南芥的 AZI1（壬二酸诱导 1）可以被壬二酸诱导，并且参与植物系统免疫的激活。在本工作中，通过 RNA 凝胶印迹分析研究了 AZI1 对低温的响应表达。低温胁迫可以缓慢诱导 AZI1 的表达，并且需要在 4°C 下处理超过 6 小时才能检测到 mRNA 丰度的增加。然而，高表达状态不能稳定维持，当植物被转移到室温时，表达水平会下降到基础水平。为了阐明 AZI1 在抗非生物胁迫中的功能，使用该基因的过表达、RNA 干扰和 T-DNA 敲除系进行了电解质泄漏测定。AZI1 的过表达导致在冷冻损伤过程中电解质泄漏减少。相比之下，AZI1 敲低和敲除系在冷冻处理后细胞损伤的趋势增加。为了进一步验证 AZI1 对低温胁迫的潜在抗性，将 pESC-AZI1 转化到酿酒酵母细胞中，其中 AZI1 受 GAL1 启动子的控制。与含有空 pESC-URA 的酵母细胞相比，含有 AZI1 的酵母细胞在冷冻处理后的存活率明显增加。所有这些结果表明，AZI1 可能具有多功能性，并与拟南芥的耐冷性有关。
