Division of Molecular Biology and Biotechnology, Indian Agriculture Research Institute, New Delhi, 110012, India.
Department of Plant Biotechnology, MGM College of Agricultural Biotechnology, Aurangabad, 431003, India.
Appl Biochem Biotechnol. 2021 Apr;193(4):1023-1041. doi: 10.1007/s12010-020-03463-y. Epub 2020 Nov 26.
Plant heat shock transcription factors (Hsfs) play a significant role in adoption under abiotic stress conditions by modulating the expression of several stress-responsive genes. Analysis of the Hsf gene family will serve to understand the molecular mechanism which is involved in response to abiotic stress. The Ziziphus species grows in warm and dry regions and is inherently tolerant to abiotic stress conditions; thus, Ziziphus is a highly enriched source of genes conferring abiotic stress tolerance. Therefore, the present study provides a comprehensive genome-wide analysis of the Hsf gene family in Z. jujuba. Identified 21 non-redundant Hsf genes were grouped into three major classes (classes A, B, and C) based on the phylogenetic analysis. Promoter and gene ontology analysis suggested that ZjHsfs perform diverse functions in response to abiotic stress conditions. Two paralogous pairs resulting from tandem gene duplication events were identified. Also, physio-chemical properties of chromosomal locations, gene structure, motifs, and protein domain organization of Hsfs were analyzed. Real-time PCR expression analyses revealed that most of the Z. jujuba Hsf genes are differentially expressed in response to heat stress. The analysis suggested ZjHsf-2, ZjHsf-3, ZjHsf-5, ZjHsf-7, ZjHsf-8, ZjHsf-10, ZjHsf-12, ZjHsf-17, and ZjHsf-18 were the outstanding candidate genes for imparting heat stress tolerance and for future functional analysis. The present analysis laid the foundation for understanding the molecular mechanism of the Hsf gene family regulating Z. jujuba development and tolerance to abiotic stress conditions.
植物热休克转录因子（Hsfs）在调节几种应激响应基因的表达方面，在适应非生物胁迫条件方面起着重要作用。对 Hsf 基因家族的分析将有助于理解参与非生物胁迫响应的分子机制。枣属植物生长在温暖和干燥的地区，天生耐受非生物胁迫条件；因此，枣属植物是赋予非生物胁迫耐受性的基因的丰富来源。因此，本研究提供了枣属植物 Hsf 基因家族的全基因组综合分析。根据系统发育分析，鉴定出 21 个非冗余 Hsf 基因，分为三大类（A、B 和 C 类）。启动子和基因本体分析表明，ZjHsfs 在应对非生物胁迫条件下具有多种功能。鉴定出两个由串联基因复制事件产生的平行基因对。此外，还分析了 Hsfs 的染色体位置、基因结构、基序和蛋白质结构域组织的理化性质。实时 PCR 表达分析显示，大多数枣属 Hsf 基因在热胁迫下的表达存在差异。分析表明，ZjHsf-2、ZjHsf-3、ZjHsf-5、ZjHsf-7、ZjHsf-8、ZjHsf-10、ZjHsf-12、ZjHsf-17 和 ZjHsf-18 是赋予耐热性和未来功能分析的候选基因。本分析为理解 Hsf 基因家族调控枣属植物发育和耐受非生物胁迫条件的分子机制奠定了基础。
