Wang Xiao-Yan, Li Dian-Zhen, Li Qi, Ma Yan-Qin, Yao Jing-Wen, Huang Xuan, Xu Zi-Qin
Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, People's Republic of China.
Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, People's Republic of China.
Plant Physiol Biochem. 2016 Oct;107:273-287. doi: 10.1016/j.plaphy.2016.06.016. Epub 2016 Jun 15.
The function of AZI1 in systemic acquired resistance of Arabidopsis was confirmed by investigation of the phenotypic features of wild-type Col-0, AZI1 T-DNA knockout and AZI1 overexpressing plants after infection with virulent and avirulent Pseudomonas syringae. Real-time quantitative PCR and Northern blotting analyses showed that the transcript abundances of PR genes increased significantly in local and systemic leaves of wild-type Col-0 and AZI1 overexpressing plants challenged with avirulent P. syringae, whereas the mRNA accumulation of PR genes was obviously attenuated in local and systemic leaves of AZI1 T-DNA knockout plants after localized infiltration with avirulent Psm avrRpm1. The changes of metabolomic profiles in distal leaves of three types of materials infected with avirulent P. syringae were determined by (1)H NMR spectrometry and data mining showed that the soluble carbonhydrates might function as signal substances in the systemic immunity of Arabidopsis. At the same time, the expression of the sugar signaling genes in local and distal leaves after infection of avirulent P. syringae was compared. As a result, it was found that the transcript abundances of sugar signaling genes, including SUS1, SUS2, SUS3, SUS6, SUT1, HXK1, HXK2, SNRK1.2, ERD6, TPS1, TOR, SNRK1.1, SNRK1.3 and bZIP11, were obviously changed in distal leaves of different materials with the modulated AZI1 activities, indicating sugar-related genes are involved in regulation of the systemic immunity mediated by AZI1. These results also illustrated that the immune system associated with sugar molecules probably was an important part of the systemic acquired resistance in Arabidopsis.
通过对野生型Col-0、AZI1 T-DNA敲除植株和AZI1过表达植株接种毒性和无毒丁香假单胞菌后的表型特征进行研究，证实了AZI1在拟南芥系统获得性抗性中的功能。实时定量PCR和Northern印迹分析表明，无毒丁香假单胞菌攻击后，野生型Col-0和AZI1过表达植株的局部和系统叶片中PR基因的转录丰度显著增加，而在用无毒Psm avrRpm1进行局部浸润后，AZI1 T-DNA敲除植株的局部和系统叶片中PR基因的mRNA积累明显减弱。通过1H NMR光谱法测定了三种材料感染无毒丁香假单胞菌后远端叶片代谢组学谱的变化，数据挖掘表明可溶性碳水化合物可能在拟南芥的系统免疫中作为信号物质发挥作用。同时，比较了无毒丁香假单胞菌感染后局部和远端叶片中糖信号基因的表达。结果发现，包括SUS1、SUS2、SUS3、SUS6、SUT1、HXK1、HXK2、SNRK1.2、ERD6、TPS1、TOR、SNRK1.1、SNRK1.3和bZIP11在内的糖信号基因的转录丰度在不同材料的远端叶片中随着AZI1活性的调节而明显变化，表明糖相关基因参与了由AZI1介导的系统免疫调节。这些结果还表明，与糖分子相关的免疫系统可能是拟南芥系统获得性抗性的重要组成部分。
