Department of Otolaryngology, University of Florida, Gainesville, FL, USA.
Otolaryngol Head Neck Surg. 2011 Sep;145(3):470-5. doi: 10.1177/0194599811405285.
Tympanostomy tube (TT) biofilm formation may lead to refractory otorrhea and occlusion. Biofilms are commonly composed of multiple microbial species. One species may promote or inhibit biofilm formation by other species.The aim of this study was to determine if Haemophilus influenzae(HI) promotes the development of Pseudomonas aeruginosa(PA) biofilm on TTs.
Controlled, in vitro.
Academic research laboratory.
Fluoroplastic TTs (20 per group) were exposed to plasma, allowed to dry, and cultured with HI for 7 days. TTs were either gas sterilized or treated for 24 hours with 10 or 3000 μg/mL ciprofloxacin. Half of the TTs from each treatment group underwent bacterial counts or scanning electron microscopy. The remainder, as well as TTs not exposed to HI, were cultured with PA for 4 days and treated with gentamicin to kill planktonic PA. Biofilm formation was quantified with bacterial counts.
TTs treated with ciprofloxacin 3000 μg/mL had lower HI counts than TTs treated with 10 μg/mL (P = .0001), but viable HI persisted. PA biofilm formation on TTs with prior HI biofilm and treated with ciprofloxacin 10 μg/mL or gas sterilization was not different than TTs without HI. Less PA biofilm formed on TTs with HI treated with 3 mg/mL ciprofloxacin(P = .002).
HI biofilm does not promote PA biofilm formation on TTs. Use of high-dose ototopical therapy to clear HI may reduce subsequent PA biofilm formation.
鼓室置管（TT）生物膜的形成可能导致难治性耳漏和堵塞。生物膜通常由多种微生物物种组成。一种物种可能通过其他物种促进或抑制生物膜的形成。本研究旨在确定流感嗜血杆菌（HI）是否促进铜绿假单胞菌（PA）在 TT 上形成生物膜。
对照，体外。
学术研究实验室。
氟塑料 TT（每组 20 个）暴露于等离子体中，干燥后与 HI 培养 7 天。TT 要么进行气体灭菌，要么用 10 或 3000μg/ml 环丙沙星处理 24 小时。每组 TT 的一半进行细菌计数或扫描电子显微镜检查。其余 TT 以及未暴露于 HI 的 TT 与 PA 一起培养 4 天，并使用庆大霉素杀死浮游 PA。生物膜形成通过细菌计数来量化。
用 3000μg/ml 环丙沙星处理的 TT 中 HI 计数低于用 10μg/ml 环丙沙星处理的 TT（P=0.0001），但存活的 HI 仍然存在。在 TT 上用庆大霉素处理前有 HI 生物膜且用 10μg/ml 环丙沙星或气体灭菌的 PA 生物膜形成与无 HI 的 TT 无差异。用 3mg/ml 环丙沙星处理 HI 的 TT 上形成的 PA 生物膜较少（P=0.002）。
HI 生物膜不会促进 TT 上的 PA 生物膜形成。使用高剂量的局部耳用抗生素清除 HI 可能会减少随后的 PA 生物膜形成。
