Ojano-Dirain Carolyn P, Silva Rodrigo C, Antonelli Patrick J
Department of Otolaryngology, University of Florida, Gainesville, FL 32610-0264, USA.
Int J Pediatr Otorhinolaryngol. 2013 Feb;77(2):223-7. doi: 10.1016/j.ijporl.2012.10.027. Epub 2012 Nov 30.
Tympanostomy tube (TT) surface modifications have been promoted as a means of reducing biofilm formation, otorrhea, and occlusion. The goal of this study was to determine if biofilm formation on silicone TTs could be prevented by commercially available surface coatings.
Silicone TTs with and without polyvinylpyrrolidone (PVP) or/and silver oxide were exposed to human plasma and cultured with Pseudomonas aeruginosa or Staphylococcus aureus (22 TTs per group). After 4 days, antibiotics were added to kill planktonic bacteria. Biofilm formation was assessed by quantitative bacterial counts and scanning electron microscopy.
PVP, silver, and PVP-silver coatings reduced P. aeruginosa biofilm formation relative to silicone by over 1 log (p<0.0001). PVP was superior to silver (p=0.04) and PVP-silver (p<0.0001). PVP and PVP-silver coatings increased S. aureus biofilm formation nominally (p=0.01 & 0.003).
PVP and silver coatings reduce P. aeruginosa biofilm formation on silicone TTs. Combining PVP and silver coatings does not further improve biofilm resistance. TT surface coatings warrant further study through clinical trials.
鼓膜造孔管（TT）表面改性已被推广为一种减少生物膜形成、耳漏和堵塞的方法。本研究的目的是确定市售表面涂层能否防止硅胶TT上生物膜的形成。
将有无聚乙烯吡咯烷酮（PVP）或/和氧化银的硅胶TT暴露于人体血浆中，并用铜绿假单胞菌或金黄色葡萄球菌培养（每组22个TT）。4天后，添加抗生素以杀死浮游细菌。通过定量细菌计数和扫描电子显微镜评估生物膜的形成。
相对于硅胶，PVP、银和PVP-银涂层使铜绿假单胞菌生物膜形成减少超过1个对数（p<0.0001）。PVP优于银（p=0.04）和PVP-银（p<0.0001）。PVP和PVP-银涂层使金黄色葡萄球菌生物膜形成略有增加（p=0.0 l和0.003）。
PVP和银涂层可减少硅胶TT上铜绿假单胞菌生物膜的形成。将PVP和银涂层结合使用并不能进一步提高生物膜抗性。TT表面涂层值得通过临床试验进行进一步研究。
