Malaty John, Antonelli Patrick J
Department of Otolaryngology, University of Florida, Gainesville, Florida 32610-0264, USA.
Laryngoscope. 2008 May;118(5):867-70. doi: 10.1097/MLG.0b013e3181671b02.
OBJECTIVES/HYPOTHESIS: Tympanostomy tube (TT) biofilm formation may lead to refractory otorrhea and occlusion. The aim of this study was to determine whether TT biofilm formation may be promoted by mucus or blood exposure.
In vitro, controlled.
Fluoroplastic TTs were exposed to blood, mucoid effusion, or saline. Half were allowed to dry. TTs were cultured with Pseudomonas aeruginosa. After 4 days, gentamicin was added to kill planktonic bacteria. Biofilm formation was assessed by quantitative bacterial counts and scanning electron microscopy.
Mucus pretreatment (dry and wet) did not increase biofilm formation. Both dry and wet blood exposure increased biofilm formation by bacterial counts (P < .0001). Biofilm formation was demonstrated by electron microscopy in all groups.
P. aeruginosa biofilm formation on fluoroplastic TTs is enhanced by blood exposure. Care should be taken to minimize bleeding with TT placement to reduce the risk of biofilm formation.
目的/假设：鼓膜置管（TT）生物膜形成可能导致难治性耳漏和堵塞。本研究的目的是确定黏液或血液暴露是否会促进TT生物膜形成。
体外对照研究。
将氟塑料TT暴露于血液、黏液样积液或盐水中。一半使其干燥。将TT与铜绿假单胞菌一起培养。4天后，加入庆大霉素以杀死浮游细菌。通过定量细菌计数和扫描电子显微镜评估生物膜形成。
黏液预处理（干燥和湿润）未增加生物膜形成。干燥和湿润血液暴露均通过细菌计数增加了生物膜形成（P <.0001）。通过电子显微镜在所有组中均证实了生物膜形成。
血液暴露可增强氟塑料TT上铜绿假单胞菌生物膜的形成。放置TT时应注意尽量减少出血，以降低生物膜形成的风险。
