Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
Mar Biotechnol (NY). 2011 Dec;13(6):1086-91. doi: 10.1007/s10126-011-9371-x. Epub 2011 Apr 15.
Toll-like receptors (TLRs) are responsible for the recognition of specific pathogen-associated molecular patterns and consequently activate signal pathways leading to inflammatory and interferon responses. The region surrounding several TLRs was previously found to be associated with resistance to specific disease. Hence, we determined the location of 11 TLRs in Japanese flounder (Paralichthys olivaceus) using polymorphic microsatellite markers. TLR1 and TLR3 were located on linkage group (LG) 21 and 7, respectively. Membrane TLR5 and soluble TLR5 were mapped to LG22. TLR7 and TLR8 were mapped to LG3. TLR9 was found on LG1 and TLR14 and TLR21 were located on the same linkage group, LG10. TLR22 was found on LG8. Interestingly, TLR2 was mapped with the previously reported Poli9-8TUF microsatellite marker which is tightly associated with lymphocystis virus disease resistance. Therefore, TLR2 is a candidate gene for resistance to lymphocystis disease. These results imply that the location of a TLR associated with a particular disease may be valuable for the research on the relationship between host immune response and disease resistance.
Toll 样受体 (TLRs) 负责识别特定的病原体相关分子模式，从而激活导致炎症和干扰素反应的信号通路。先前发现，TLRs 周围的区域与对特定疾病的抗性有关。因此，我们使用多态性微卫星标记物确定了日本牙鲆 (Paralichthys olivaceus) 中的 11 个 TLR 的位置。TLR1 和 TLR3 分别位于连锁群 (LG) 21 和 7 上。膜 TLR5 和可溶性 TLR5 被映射到 LG22。TLR7 和 TLR8 被映射到 LG3。TLR9 位于 LG1 上，TLR14 和 TLR21 位于同一连锁群 LG10 上。TLR22 位于 LG8 上。有趣的是，TLR2 与先前报道的与淋巴囊肿病毒病抗性紧密相关的 Poli9-8TUF 微卫星标记物一起被定位。因此，TLR2 是淋巴囊肿病抗性的候选基因。这些结果表明，与特定疾病相关的 TLR 的位置对于研究宿主免疫反应与疾病抗性之间的关系可能具有重要价值。
