García-Angulo Aglaya, Merlo Manuel A, Rodríguez María E, Portela-Bens Silvia, Liehr Thomas, Rebordinos Laureana
Área de Genética, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Cádiz, Spain.
Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University Jena, Jena, Germany.
Front Genet. 2019 Jun 11;10:529. doi: 10.3389/fgene.2019.00529. eCollection 2019.
Global aquaculture production continues to increase rapidly. One of the most important species of marine fish currently cultivated in Southern Europe is , reaching more than 300 Tn in 2017. In the present work, 14 Bacterial Artificial Chromosome (BAC) clones containing candidate genes involved in the immune system (, , , , α, , , , , , , , and ), were examined and compared with other species using multicolor Fluorescence Hybridization (mFISH), massive sequencing and bioinformatic analysis to determine the genomic surroundings and syntenic chromosomal conservation of the genomic region contained in each BAC clone. The mFISH showed that the groups of genes ; ; , and were co-localized on the same chromosomes. Synteny results suggested that the studied BACs are placed in a smaller number of chromosomes in that in other species. Phylogenetic analyses suggested that the evolutionary rate of immune system genes studied is similar among the taxa studied, given that the clustering obtained was in accordance with the accepted phylogenetic relationships among these species. This study contributes to a better understanding of the structure and function of the immune system of the Senegalese sole, which is essential for the development of new technologies and products to improve fish health and productivity.
全球水产养殖产量持续快速增长。目前在南欧养殖的最重要的海水鱼类品种之一是 ，2017年产量超过300吨。在本研究中，对14个包含参与免疫系统的候选基因（ 、 、 、 、α、 、 、 、 、 、 、 、 和 ）的细菌人工染色体（BAC）克隆进行了检测，并使用多色荧光原位杂交（mFISH）、大规模测序和生物信息学分析与其他物种进行比较，以确定每个BAC克隆中所含基因组区域的基因组环境和同线染色体保守性。mFISH结果表明，基因组 ； ； 以及 共定位于同一条染色体上。同线分析结果表明，与其他物种相比，所研究的BAC在 中分布于较少数量的染色体上。系统发育分析表明，鉴于所获得的聚类与这些物种之间公认的系统发育关系一致，在所研究的分类群中，所研究的免疫系统基因的进化速率相似。本研究有助于更好地理解塞内加尔鳎免疫系统的结构和功能，这对于开发改善鱼类健康和生产力的新技术和产品至关重要。
