Department of Food Science, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.
J Agric Food Chem. 2011 May 25;59(10):5305-11. doi: 10.1021/jf200180n. Epub 2011 Apr 26.
This study determined the in vitro inhibitory effects of cocoa extracts and procyanidins against pancreatic α-amylase (PA), pancreatic lipase (PL), and secreted phospholipase A(2) (PLA(2)) and characterized the kinetics of such inhibition. Lavado, regular, and Dutch-processed cocoa extracts as well as cocoa procyanidins (degree of polymerization (DP) = 2-10) were examined. Cocoa extracts and procyanidins dose-dependently inhibited PA, PL, and PLA(2). Lavado cocoa extract was the most potent inhibitor (IC(50) = 8.5-47 μg/mL). An inverse correlation between log IC(50) and DP (R(2) > 0.93) was observed. Kinetic analysis suggested that regular cocoa extract, the pentamer, and decamer inhibited PL activity in a mixed mode. The pentamer and decamer noncompetitively inhibited PLA(2) activity, whereas regular cocoa extract inhibited PLA(2) competitively. This study demonstrates that cocoa polyphenols can inhibit digestive enzymes in vitro and may, in conjunction with a low-calorie diet, play a role in body weight management.
本研究旨在确定可可提取物和原花青素对胰α-淀粉酶（PA）、胰脂肪酶（PL）和分泌型 PLA2（PLA2）的体外抑制作用，并对其抑制动力学进行表征。研究考察了水洗可可提取物、常规可可提取物、荷兰加工可可提取物以及可可原花青素（聚合度 DP = 2-10）。可可提取物和原花青素可剂量依赖性抑制 PA、PL 和 PLA2。水洗可可提取物的抑制作用最强（IC50 = 8.5-47 μg/mL）。观察到 log IC50 与 DP 之间呈负相关（R2 > 0.93）。动力学分析表明，常规可可提取物、五聚体和十聚体以混合模式抑制 PL 活性。五聚体和十聚体非竞争性抑制 PLA2 活性，而常规可可提取物则竞争性抑制 PLA2。本研究表明，可可多酚可在体外抑制消化酶，可能与低热量饮食一起在体重管理中发挥作用。
