基于微流控技术的生物分子 18F 标记用于免疫正电子发射断层扫描。
Microfluidic-based 18F-labeling of biomolecules for immuno-positron emission tomography.
机构信息
College of Electronics and Information Engineering, Wuhan Textile University, Wuhan, China.
出版信息
Mol Imaging. 2011 Jun;10(3):168-76, 1-7.
Abstract
Methods for tagging biomolecules with fluorine 18 as immuno-positron emission tomography (immunoPET) tracers require tedious optimization of radiolabeling conditions and can consume large amounts of scarce biomolecules. We describe an improved method using a digital microfluidic droplet generation (DMDG) chip, which provides computer-controlled metering and mixing of 18F tag, biomolecule, and buffer in defined ratios, allowing rapid scouting of reaction conditions in nanoliter volumes. The identified optimized conditions were then translated to bench-scale 18F labeling of a cancer-specific engineered antibody fragments, enabling microPET imaging of tumors in xenografted mice at 0.5 to 4 hours postinjection.
摘要
方法用氟 18 标记生物分子作为免疫正电子发射断层扫描（immunoPET）示踪剂需要繁琐的优化放射性标记条件，并消耗大量的稀缺生物分子。我们描述了一种改进的方法，使用数字微流控液滴生成（DMDG）芯片，它提供了计算机控制的计量和混合 18F 标签，生物分子和缓冲液以定义的比例，允许快速侦察反应条件在纳升体积。确定的优化条件，然后转化为台式 18F 标记的癌症特异性工程抗体片段，使肿瘤微 PET 成像在异种移植小鼠在 0.5 至 4 小时后注射。
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