Lesnikova E V, Sabitov E A, Strelkova M V, Shurkhal A V, Rakitskaia T A
Med Parazitol (Mosk). 1990 Nov-Dec(6):6-9.
Using electrophoresis in polyacrylamide gel, 11 isolates have been detected by 8 enzymes (PGI, PGM, 6-PGD, MDG, G-6-PGD, ME, ALAT, ASAT) from patients with visceral leishmaniasis and dogs in the Turkmen SSR. The isolates were obtained from different areas of the republic varying in landscape and climatic conditions. The isolates were maintained by passages on golden hamsters. No long-lasting Leishmania culture has been obtained on the nutrition medium for any of the isolates. Parasite biomass was obtained by cultivation of spleen and liver suspensions on the nutrition medium. All the isolates were identified as Leishmania infantum, representing, however, independent zymodems. Isolates from human patients identified by PGI in the foci of the southeastern Kara Kum were distinct from those isolated from human patients in the foci of western Kopet-Dag. The isolates from 1-2-year-old children and an adult patient aged 31 in the same area were identical. The isolates from dogs were different from human ones in PGI and were not identical with Lem 78 marker isolated from dogs.
利用聚丙烯酰胺凝胶电泳，通过8种酶（磷酸葡萄糖异构酶、磷酸葡萄糖变位酶、6-磷酸葡萄糖酸脱氢酶、苹果酸脱氢酶、6-磷酸葡萄糖酸脱氢酶、苹果酸酶、丙氨酸转氨酶、天冬氨酸转氨酶）对土库曼苏维埃社会主义共和国内脏利什曼病患者和犬类的11株分离株进行了检测。这些分离株取自该共和国不同地区，这些地区的地形和气候条件各不相同。分离株通过在金黄仓鼠身上传代得以保存。对于任何一株分离株，均未在营养培养基上获得持久的利什曼原虫培养物。通过在营养培养基上培养脾脏和肝脏悬液获得寄生虫生物量。所有分离株均被鉴定为婴儿利什曼原虫，但代表不同的酶带型。在卡拉库姆沙漠东南部疫源地，通过磷酸葡萄糖异构酶鉴定的来自人类患者的分离株与在科佩特山脉西部疫源地从人类患者中分离出的菌株不同。同一地区1至2岁儿童和一名31岁成年患者的分离株相同。犬类分离株在磷酸葡萄糖异构酶方面与人类分离株不同，且与从犬类分离出的Lem 78标记物不同。
