National Forensic Service, 331-1 Shinwol 7-Dong, Yang-Chun Gu, Seoul, South Korea.
Forensic Sci Int. 2011 Jul 15;210(1-3):201-5. doi: 10.1016/j.forsciint.2011.03.017. Epub 2011 Apr 16.
In this study, we investigated the patterns of cannabis users (n=412) according to their sex, age, and the results of urinalysis and hair analysis, and classified the concentrations of THCCOOH in hair into three categories to examine the levels of cannabis use. We also compared the concentrations of THCCOOH in hair root, hair without the hair root and whole hair and examined the relationship among them according to the results of urinalysis. The hair samples were washed, digested with 1ml of 1M NaOH at 85°C for 30min and extracted with 2ml of n-hexane:ethyl acetate (9:1) two times after adding 1ml of 0.1N sodium acetate buffer (pH 4.5) and 200μl of acetic acid. The final mixture was derivatized with 50μl of PFPA and 25μl of PFPOH for 30min at 70°C. The solution was evaporated, and the residue was reconstituted in 40μl of ethyl acetate and transferred to an autosampler vial. One microlitre was injected into the GC/MS/MS-NCI system. The concentrations of THCCOOH ranged from 0.06 to 33.44pg/mg (mean 2.96; median 1.32) in hair from cannabis users who had positive urine results and ranged from 0.05 to 7.24pg/mg (mean 1.35; median 0.37) in hair from cannabis users who had negative urine results. The average concentration of THCCOOH in hair from cannabis users who had positive urine results was higher than that from cannabis users who had negative urine results. Male cannabis users in their forties were predominant. We classified the concentrations of THCCOOH in hair into three groups (low, medium and high), and could use the grouping of THCCOOH in hair as a guide for determining the level of use. The low, medium and high concentration ranges for THCCOOH in hair were 0.05-0.24, 0.25-2.60 and 2.63-33.44pg/mg, respectively. We also investigated 28 hair samples with the root. The highest concentrations of THCCOOH were seen in the hair root from 18 out of the 28 hair samples. The average concentrations of THCCOOH in hair root, hair without hair root and whole hair from cannabis users who had positive urine results were higher than those who had negative urine results.
在这项研究中，我们根据性别、年龄以及尿液分析和毛发分析的结果，对 412 名大麻使用者的模式进行了调查，并将毛发中 THCCOOH 的浓度分为三个类别，以检查大麻使用的水平。我们还比较了毛发根部、无毛发根部的毛发和整个毛发中的 THCCOOH 浓度，并根据尿液分析的结果检查了它们之间的关系。毛发样本先用 1ml1MNaOH 在 85°C 下清洗 30min，然后加入 1ml0.1N 乙酸钠缓冲液(pH4.5)和 200μl 乙酸后，用 2ml 正己烷:乙酸乙酯(9:1)提取两次。最后将混合物与 50μlPFPA 和 25μlPFPOH 在 70°C 下衍生 30min。将溶液蒸发，残留物用 40μl 乙酸乙酯重新配制，转移到自动进样瓶中。将 1μl 注入 GC/MS/MS-NCI 系统。尿液检测呈阳性的大麻使用者毛发中 THCCOOH 的浓度范围为 0.06 至 33.44pg/mg（平均值 2.96；中位数 1.32），尿液检测呈阴性的大麻使用者毛发中 THCCOOH 的浓度范围为 0.05 至 7.24pg/mg（平均值 1.35；中位数 0.37）。尿液检测呈阳性的大麻使用者毛发中 THCCOOH 的平均浓度高于尿液检测呈阴性的大麻使用者。四十多岁的男性大麻使用者居多。我们将毛发中 THCCOOH 的浓度分为三组（低、中、高），并可以将毛发中 THCCOOH 的分组作为确定使用水平的指导。毛发中 THCCOOH 的低、中、高浓度范围分别为 0.05-0.24、0.25-2.60 和 2.63-33.44pg/mg。我们还对 28 个带根的毛发样本进行了调查。在 28 个毛发样本中，有 18 个毛发样本的根中 THCCOOH 的浓度最高。尿液检测呈阳性的大麻使用者毛发根部、无毛发根部的毛发和整个毛发中的 THCCOOH 平均浓度高于尿液检测呈阴性的大麻使用者。
