National Forensic Service, 331-1 Shinwol 7-Dong, Yang-Chun Gu, Seoul, South Korea.
Forensic Sci Int. 2011 Oct 10;212(1-3):238-41. doi: 10.1016/j.forsciint.2011.06.026. Epub 2011 Jul 29.
In this study, the concentrations of 11-nor-Δ(9)-tetrahydrocannabinol-9-carboxylic acid (THCCOOH) in pubic, axillary and beard hair were measured and the correlation between the concentrations of THCCOOH in head and pubic hair from same cannabis users were evaluated. The papers on body hair analysis for THCCOOH were rarely found although police officers submit body hair as a complimentary specimen to forensic laboratories in case cannabis users had no hair. Head, pubic, axillary, and beard hair were collected. All hair samples were cut into 0.5mm segments and decontaminated with methanol, digested with 1 mL of 1M NaOH at 85 °C for 30 min and extracted in 2 mL of n-hexane:ethyl acetate (9:1) two times after adding 1 mL of 0.1N sodium acetate buffer (pH = 4.5) and 200 μL of acetic acid followed by derivatization with 50 μL of PFPA and 25 μL of PFPOH for 30 min at 70 °C. The extracts were analyzed using gas chromatography tandem mass spectrometry operating in negative chemical ionization mode (GC/MS/MS-NCI). We determined the concentrations of THCCOOH in both pubic and head hair. The concentrations of THCCOOH in pubic hair were higher than those in head hair. We also evaluated the concentrations of THCCOOH in body hair (pubic, axillary and beard hair) and head hair according to the positive/negative urine test results. There was no statistically significant difference in the concentrations of THCCOOH in head and body hair according to urine results.
在这项研究中，测量了 11-去甲-Δ(9)-四氢大麻酸-9-羧酸（THCCOOH）在阴毛、腋毛和胡须中的浓度，并评估了来自同一大麻使用者的头部和阴毛中 THCCOOH 浓度之间的相关性。尽管警察在没有毛发的情况下将体毛作为补充样本提交给法医实验室，但很少有关于体毛中 THCCOOH 分析的论文。收集了头发、阴毛、腋毛和胡须。所有毛发样本均切成 0.5mm 段，用甲醇进行脱污染，在 85°C 下用 1ml1M NaOH 消化 30 分钟，然后在加入 1ml0.1N 乙酸钠缓冲液（pH=4.5）和 200μl 乙酸后，用 2ml 正己烷：乙酸乙酯（9：1）提取两次，并在 70°C 下用 50μlPFPA 和 25μlPFPOH 衍生 30 分钟。使用在负离子化学电离模式下运行的气相色谱串联质谱（GC/MS/MS-NCI）分析提取物。我们测定了阴毛和头发中 THCCOOH 的浓度。阴毛中的 THCCOOH 浓度高于头发中的 THCCOOH 浓度。我们还根据尿液检测结果评估了体毛（阴毛、腋毛和胡须）和头部毛发中 THCCOOH 的浓度。根据尿液结果，头部和体毛中 THCCOOH 的浓度没有统计学差异。
