National Forensic Service, 111 Daedeok-ri, Seosam-myeon, Jangseong-gun, Jeollanam-do 515-822, Korea.
J Anal Toxicol. 2012 Apr;36(3):195-200. doi: 10.1093/jat/bks010.
Cannabis is the most widely abused drug in the world. The purpose of this study is to detect 11-nor-9-carboxy-Δ⁹-tetrahydrocannabinol (THCCOOH) in segmental hair and to evaluate the patterns of cannabis use. We investigated the relationship between the concentrations of THCCOOH in hair and the self-reported use data and the route of administration. For this purpose, the hair samples were washed, digested with 1 mL of 1 M NaOH at 85°C for 30 min along with the internal standard, THCCOOH-d₃ (2.5 pg/mg) and extracted in 2 mL of n-hexane-ethyl acetate (9:1) twice after adding 1 mL of 0.1N sodium acetate buffer (pH = 4.5) and 200 µL of acetic acid. The organic extract was transferred and evaporated and the mixture was derivatized with 50 µL of pentafluoropropionic anhydride and 25 µL of pentafluoropropanol for 30 min at 70°C. Reconstituted final extract was injected into the gas chromatography-tandem mass spectrometer operating in the negative chemical ionization mode. In segmental hair analysis, the concentrations of THCCOOH decreased from the proximal to distal segments. The concentrations of THCCOOH in hair and the self-reported dose and frequency of administration from cannabis users were not well correlated because of the low accuracy and reliability of the self-reported data. However, this study provides preliminary information on the dose and frequency of administration among cannabis users in our country.
大麻是世界上滥用最广泛的毒品。本研究旨在检测毛发中的 11-去甲-9-羧基-Δ⁹-四氢大麻酚（THCCOOH），并评估大麻使用模式。我们调查了毛发中 THCCOOH 浓度与自我报告的使用数据和给药途径之间的关系。为此，将毛发样品用 1 mL 1 M NaOH 在 85°C 下加热 30 分钟进行处理，同时加入内标物 THCCOOH-d₃（2.5 pg/mg），并在加入 1 mL 0.1N 乙酸钠缓冲液（pH = 4.5）和 200 µL 乙酸后，用 2 mL 正己烷-乙酸乙酯（9:1）分两次进行提取。将有机提取物转移并蒸发，并用 50 µL 五氟丙酸酐和 25 µL 五氟丙醇在 70°C 下衍生 30 分钟。将重新配制的最终提取物注入在负离子化学电离模式下运行的气相色谱-串联质谱仪中。在分段毛发分析中，THCCOOH 的浓度从近端到远端逐渐降低。由于自我报告数据的准确性和可靠性较低，毛发中 THCCOOH 的浓度与大麻使用者自我报告的剂量和给药频率之间没有很好的相关性。然而，本研究提供了有关我国大麻使用者给药剂量和频率的初步信息。
