Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
Database (Oxford). 2011 Apr 15;2011:bar006. doi: 10.1093/database/bar006. Print 2011.
Laccases and their homologues form the protein superfamily of multicopper oxidases (MCO). They catalyze the oxidation of many, particularly phenolic substances, and, besides playing an important role in many cellular activities, are of interest in biotechnological applications. The Laccase Engineering Database (LccED, http://www.lcced.uni-stuttgart.de) was designed to serve as a tool for a systematic sequence-based classification and analysis of the diverse multicopper oxidase protein family. More than 2200 proteins were classified into 11 superfamilies and 56 homologous families. For each family, the LccED provides multiple sequence alignments, phylogenetic trees and family-specific HMM profiles. The integration of structures for 14 different proteins allows a comprehensive comparison of sequences and structures to derive biochemical properties. Among the families, the distribution of the proteins regarding different kingdoms was investigated. The database was applied to perform a comprehensive analysis by MCO- and laccase-specific patterns. The LccED combines information of sequences and structures of MCOs. It serves as a classification tool to assign new proteins to a homologous family and can be applied to investigate sequence-structure-function relationship and to guide protein engineering. Database URL: http://www.lcced.uni-stuttgart.de.
漆酶及其同源物形成多铜氧化酶（MCO）的蛋白质超家族。它们催化许多物质的氧化，特别是酚类物质，除了在许多细胞活动中发挥重要作用外，在生物技术应用中也具有重要意义。漆酶工程数据库（LccED，http://www.lcced.uni-stuttgart.de）旨在作为一种基于序列的系统分类和分析多样化的多铜氧化酶蛋白家族的工具。超过 2200 种蛋白质被分类为 11 个超家族和 56 个同源家族。对于每个家族，LccED 提供了多个序列比对、系统发育树和家族特异性 HMM 图谱。14 种不同蛋白质的结构整合允许对序列和结构进行全面比较，以得出生化性质。在这些家族中，研究了不同王国的蛋白质分布情况。该数据库被用于通过 MCO 和漆酶特异性模式进行全面分析。LccED 结合了 MCO 的序列和结构信息。它作为一种分类工具，将新的蛋白质分配到同源家族中，并可用于研究序列-结构-功能关系和指导蛋白质工程。数据库网址：http://www.lcced.uni-stuttgart.de。
