Mount Sinai Medical Center, Box 1236, New York 10029, USA.
Anticancer Res. 2011 Mar;31(3):959-60.
Glioblastoma multiforme is the most common and most aggressive type of primary brain tumor, accounting for 52% of all primary brain tumor cases and 20% of all intracranial tumors. Recently, evidence for a viral cause has been postulated, possibly SV40 or more likely cytomegalovirus (CMV). One report indicated that 80% of patients with newly diagnosed glioblastoma multiforme have detectable cytomegalovirus DNA in their peripheral blood, while sero-positive normal donors and other surgical patients did not exhibit detectable virus.
In the current study, we examined peripheral blood of 5 patients with newly diagnosed glioblastoma multiforme. Peripheral blood was collected in anticoagulated tubes from five patients with newly diagnosed glioblastoma multiforme referred for radiation therapy. We used standard methods for detecting CMV by reverse transcriptase-polymerase chain reaction (RT-PCR) and peripheral blood culture.
None of our patients had circulating CMV.
There are four subtypes of glioblastoma. We hypothesize that circulating CMV might be limited to some, but not all of these subtypes, and that our failure to detect CMV might be attributed to the fact that none of these patients had the appropriate subtype or subtypes.
多形性胶质母细胞瘤是最常见和最具侵袭性的原发性脑肿瘤，占所有原发性脑肿瘤病例的 52%，占所有颅内肿瘤的 20%。最近，有人提出病毒是其病因，可能是 SV40 或更可能是巨细胞病毒（CMV）。有一份报告表明，80%新诊断为多形性胶质母细胞瘤的患者的外周血中可检测到巨细胞病毒 DNA，而血清阳性的正常供体和其他手术患者则未检测到病毒。
在本研究中，我们检测了 5 例新诊断为多形性胶质母细胞瘤患者的外周血。从 5 例新诊断为多形性胶质母细胞瘤并接受放射治疗的患者的抗凝管中采集外周血。我们使用逆转录-聚合酶链反应（RT-PCR）和外周血培养标准方法检测 CMV。
我们的患者均未检测到循环 CMV。
多形性胶质母细胞瘤有四个亚型。我们假设循环 CMV 可能局限于其中一些，但不是所有这些亚型，我们未能检测到 CMV 可能是因为这些患者没有适当的亚型或亚型。
