Chen Ning, Hudson Jennifer E, Walczak Piotr, Misiuta Iwona, Garbuzova-Davis Svitlana, Jiang Lixian, Sanchez-Ramos Juan, Sanberg Paul R, Zigova Tanja, Willing Alison E
Center of Excellence for Aging and Brain Repair, Department of Neurosurgery, University of South Florida, College of Medicine, Tampa, Florida 33612, USA.
Stem Cells. 2005 Nov-Dec;23(10):1560-70. doi: 10.1634/stemcells.2004-0284. Epub 2005 Aug 4.
The mononuclear fraction from human umbilical cord blood (HUCB) contains a significant number of stem/progenitor cells that in theory could be come any cell in the body, including neurons. Taking into consideration that transdifferentiation would be a very rare event and also knowing that overlapping genetic programs for hematopoiesis and neuropoiesis exist, we undertook a characterization of the HUCB mononuclear fraction, including analysis of cellular subpopulations and their morphology, cell viability, proliferation, and expression of neural and hematopoietic antigens. Two cell populations were apparent-adherent and floating fractions. The adherent fraction was mainly lymphocytes (~53%) expressing hematopoietic antigens. Upon replate, the floating population had many cells that expressed stem cell antigens. More of the cells in this subfraction expressed neural proteins. Neurotrophin receptors trkB and trkC were present in both cell fractions, although expression was higher in the floating fraction. Our initial characterization suggests that a subpopulation of cells exists within the HUCB mononuclear fraction that seems to have the potential to become neural cells, which could then be used in the development of cell-based therapies for brain injuries and diseases.
人脐带血（HUCB）的单核细胞部分含有大量的干/祖细胞，理论上这些细胞可以分化为体内任何细胞，包括神经元。考虑到转分化是一个非常罕见的事件，并且也知道存在造血和神经发生的重叠遗传程序，我们对HUCB单核细胞部分进行了表征，包括细胞亚群及其形态、细胞活力、增殖以及神经和造血抗原表达的分析。明显有两个细胞群体——贴壁和悬浮部分。贴壁部分主要是表达造血抗原的淋巴细胞（约53%）。再次接种时，悬浮群体中有许多细胞表达干细胞抗原。该亚群中更多的细胞表达神经蛋白。神经营养因子受体trkB和trkC在两个细胞部分中均有存在，尽管悬浮部分的表达更高。我们的初步表征表明，HUCB单核细胞部分中存在一个细胞亚群，似乎有潜力成为神经细胞，然后可用于开发针对脑损伤和疾病的细胞疗法。
