Wilton J M, Hurst T J, Carman R J, Macey M G
Medical Research Council Dental Research Unit, London Hospital, U.K.
FEMS Microbiol Immunol. 1990 Dec;2(5-6):285-93. doi: 10.1111/j.1574-6968.1990.tb03531.x.
Porphyromonas gingivalis culture supernate was found to induce homotypic agglutination of human polymorphonuclear leukocytes (PMN). Pretreatment of PMN with P. gingivalis supernate inhibited both the rate and the degree of agglutination induced by the secretagogues PMA and FMLP. Lipopolysaccharide from P. gingivalis upregulated the CR3 (Mac-1, CD11b) receptors on PMN. Treatment of glass-adherent PMN with P. gingivalis supernate did not alter their phagocytic capacity for P. gingivalis cells but when PMN were pretreated in suspension the cells adhered less well to glass and phagocytosis of those PMN that did adhere was reduced. P. gingivalis supernate treatment of PMN induced lysozyme release but the amount released during phagocytosis when supernate was present did not change. Neither P. gingivalis supernate nor LPS were cytotoxic for PMN. The data suggest that P. gingivalis factors could interfere with PMN elimination of this organism at the site of infection by inappropriately stimulating PMN, depressing phagocytosis and causing enhanced CR3 expression. The consequent agglutination or enhanced adherence could also lead to decreased phagocytic capacity of the adherent or agglutinated cells.
牙龈卟啉单胞菌培养上清液被发现可诱导人多形核白细胞（PMN）的同型凝集。用牙龈卟啉单胞菌上清液预处理PMN可抑制由分泌刺激剂佛波酯（PMA）和N-甲酰甲硫氨酸-亮氨酸-苯丙氨酸（FMLP）诱导的凝集速率和程度。牙龈卟啉单胞菌的脂多糖上调PMN上的补体受体3（CR3，巨噬细胞抗原-1，CD11b）受体。用牙龈卟啉单胞菌上清液处理玻璃黏附的PMN不会改变其对牙龈卟啉单胞菌细胞的吞噬能力，但当PMN在悬浮液中进行预处理时，细胞与玻璃的黏附性降低，并且那些黏附的PMN的吞噬作用也减弱。用牙龈卟啉单胞菌上清液处理PMN可诱导溶菌酶释放，但当存在上清液时，吞噬过程中释放的溶菌酶量没有变化。牙龈卟啉单胞菌上清液和脂多糖对PMN均无细胞毒性。数据表明，牙龈卟啉单胞菌的因子可能通过不适当地刺激PMN、抑制吞噬作用和导致CR3表达增强，干扰感染部位该菌的PMN清除。随之而来的凝集或黏附增强也可能导致黏附或凝集细胞的吞噬能力下降。
