Shioi Go, Kiyonari Hiroshi, Abe Takaya, Nakao Kazuki, Fujimori Toshihiko, Jang Chuan-Wei, Huang Cheng-Chiu, Akiyama Haruhiko, Behringer Richard R, Aizawa Shinichi
Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology (CDB), Kobe 650-0047, Japan.
Genesis. 2011 Jul;49(7):570-8. doi: 10.1002/dvg.20758. Epub 2011 Jun 17.
Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and resolution of mCherry nuclear localization and EGFP membrane localization were demonstrated to be sufficient for live-imaging with embryos that express RG (mCherry and EGFP) ubiquitously and specifically in fetal Sertoli cells. The conditional R26R-RG reporter mouse line should be a useful tool for labeling nuclei and membranes simultaneously in distinct cell populations.
活体成像技术是观察活细胞并监测其发育过程中行为的重要工具。该技术需要多种小鼠报告基因品系，每个品系都独特地表达一种荧光蛋白。在此，我们开发了一种R26R-RG报告基因小鼠品系，它能分别从Rosa26位点在细胞核和质膜中条件性且同时地表达mCherry和EGFP。mCherry核定位和EGFP膜定位的强度和分辨率被证明足以对在胚胎中普遍且特异性地在胎儿支持细胞中表达RG（mCherry和EGFP）的胚胎进行活体成像。条件性R26R-RG报告基因小鼠品系应该是在不同细胞群体中同时标记细胞核和细胞膜的有用工具。
