小鼠胚胎中的活体成像荧光蛋白：多维、多光谱视角
Live-imaging fluorescent proteins in mouse embryos: multi-dimensional, multi-spectral perspectives.
作者信息
Nowotschin Sonja, Eakin Guy S, Hadjantonakis Anna-Katerina
机构信息
Developmental Biology Program, Sloan-Kettering Institute, New York, NY10065, USA.
出版信息
Trends Biotechnol. 2009 May;27(5):266-76. doi: 10.1016/j.tibtech.2009.02.006. Epub 2009 Mar 30.
Abstract
Microscopy has always been an obligate tool in the field of developmental biology, a goal of which is to elucidate the essential cellular and molecular interactions that coordinate the specification of different cell types and the establishment of body plans. The 2008 Nobel Prize in chemistry was awarded 'for the discovery and development of the green fluorescent protein, GFP' in recognition that the discovery of genetically encoded fluorescent proteins (FPs) has spearheaded a revolution in applications for imaging of live cells. With the development of more-sophisticated imaging technology and availability of FPs with different spectral characteristics, dynamic processes can now be live-imaged at high resolution in situ in embryos. Here, we review some recent advances in this rapidly evolving field as applied to live-imaging capabilities in the mouse, the most genetically tractable mammalian model organism for embryologists.
摘要
显微镜检查一直是发育生物学领域必不可少的工具，该领域的一个目标是阐明协调不同细胞类型的特化和身体蓝图建立的基本细胞和分子相互作用。2008年诺贝尔化学奖授予“发现和发展绿色荧光蛋白GFP”，以表彰基因编码荧光蛋白（FPs）的发现引领了活细胞成像应用的一场革命。随着更先进成像技术的发展以及具有不同光谱特征的荧光蛋白的出现，现在可以在胚胎中原位以高分辨率对动态过程进行实时成像。在这里，我们回顾一下这个快速发展的领域在小鼠（胚胎学家最易进行基因操作的哺乳动物模式生物）活体成像能力方面的一些最新进展。
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本文引用的文献
1
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Genesis. 2009 May;47(5):330-6. doi: 10.1002/dvg.20500.
2
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Nat Methods. 2009 Feb;6(2):153-9. doi: 10.1038/nmeth.1298. Epub 2009 Jan 25.
3
In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts.肿瘤相关成纤维细胞介导的细胞外基质重塑的体内成像
Nat Methods. 2009 Feb;6(2):143-5. doi: 10.1038/nmeth.1295. Epub 2009 Jan 18.
4
Optical lock-in detection imaging microscopy for contrast-enhanced imaging in living cells.用于活细胞中对比度增强成像的光学锁相检测成像显微镜。
Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):17789-94. doi: 10.1073/pnas.0808882105. Epub 2008 Nov 12.
5
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Science. 2008 Nov 14;322(5904):1065-9. doi: 10.1126/science.1162493. Epub 2008 Oct 9.
6
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10
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