Jaishree V, Badami Shrishailappa, Krishnamurthy Praveen Thaggikuppe
Department of Pharmaceutical Chemistry, J. S. S. College of Pharmacy, Ootacamund 643 001, India.
Indian J Exp Biol. 2010 Sep;48(9):896-904.
Enicostemma axillare is used in Indian traditional medicine as a liver tonic. Its ethyl acetate extract has shown potent in vitro antioxidant activity and found to contain 7.26% of a bitter secoiridoid glycoside, swertiamarin. Hence, in the present study the ethyl acetate extract was screened for hepatoprotective and antioxidant properties against CCl4 induced hepatic injury in rats. The hepatoprotection was assessed in terms of reduction in histological damage and changes in serum enzymes and metabolites. The pretreatment with the extract at 100 and 200 mg/kg body weight doses given orally for eight days prior to CCl4 caused significant restoration of altered biochemical changes due to CCl4 towards the normal in serum, liver and kidney. The extract treatment at 200 mg/kg body weight was found to be more potent than the standard silymarin at 100 mg/kg body weight in reversing most of the biochemical parameters. Histopathological studies complemented the results of biochemical estimations in providing a proof of hepatoprotective and antioxidant actions of the extract. The study provides a support to the ethnomedical use of E. axillare in India.
腋花獐牙菜在印度传统医学中用作肝脏滋补剂。其乙酸乙酯提取物已显示出强大的体外抗氧化活性，并且发现含有7.26%的苦味裂环烯醚萜苷——獐牙菜苦苷。因此，在本研究中，对乙酸乙酯提取物针对四氯化碳诱导的大鼠肝损伤的保肝和抗氧化特性进行了筛选评估。通过组织学损伤的减轻以及血清酶和代谢物的变化来评估保肝作用。在给予四氯化碳之前，以100和200mg/kg体重剂量口服提取物进行预处理8天，可使因四氯化碳导致的血清、肝脏和肾脏中生化变化的改变显著恢复至正常水平。发现在使大多数生化参数恢复正常方面，200mg/kg体重的提取物处理比100mg/kg体重的标准水飞蓟宾更有效。组织病理学研究补充了生化评估结果，为提取物的保肝和抗氧化作用提供了证据。该研究为腋花獐牙菜在印度的民族医学应用提供了支持。
