Deshpande Sunita D, Kulkarni Kirti S
Department of Microbiology, T.N.M.C & B.Y.L. Nair Hospital, Mumbai Central 400 008, India.
Indian J Exp Biol. 2010 Sep;48(9):931-5.
Staphylococcus aureus is the most frequently isolated pathogen from wounds with multiple resistances to antibiotics. Honey has been demonstrated and reported to be effective antibacterial agent on Gram positive and Gram negative organisms. Hence, the present study was conducted to evaluate the in vitro antibacterial effect of Indian honeys on Staphylococcus aureus obtained from wounds. A total of 123 Staphylococcus aureus isolates along with ATCC 25923 were categorized as sensitive, multi drug resistant (MDR) and non-MDR strains. Out of total nine Indian honeys (three each of unifloral, multifloral and branded marketed honey) used, three unifloral and three multifloral honey samples showed antibacterial activity against all the organisms tested by Agar diffusion method but not the branded marketed honeys. The MIC values of all honey samples for all studied Staphylococcus aureus isolates ranged between 5-15% (v/v). Unifloral honey samples showed higher antibacterial activity than multifloral honey. The single sample of Jambhul honey showed the highest activity. Thus, Indian honeys were found to be effective for their antimicrobial activity on sensitive, non-MDR, MDR and ATCC strains of S. aureus.
金黄色葡萄球菌是从伤口中最常分离出的对多种抗生素具有耐药性的病原体。蜂蜜已被证明并报道为对革兰氏阳性菌和革兰氏阴性菌有效的抗菌剂。因此，本研究旨在评估印度蜂蜜对从伤口分离出的金黄色葡萄球菌的体外抗菌作用。总共123株金黄色葡萄球菌分离株以及ATCC 25923被分类为敏感、多重耐药（MDR）和非MDR菌株。在所使用的总共九种印度蜂蜜（单花蜂蜜、多花蜂蜜和品牌销售蜂蜜各三种）中，三种单花蜂蜜和三种多花蜂蜜样品通过琼脂扩散法对所有测试的菌株均显示出抗菌活性，但品牌销售蜂蜜则没有。所有蜂蜜样品对所有研究的金黄色葡萄球菌分离株的最低抑菌浓度（MIC）值在5-15%（v/v）之间。单花蜂蜜样品显示出比多花蜂蜜更高的抗菌活性。Jambhul蜂蜜的单个样品显示出最高活性。因此，发现印度蜂蜜对金黄色葡萄球菌的敏感、非MDR、MDR和ATCC菌株具有抗菌活性。
