体外评估耐甲氧西林和甲氧西林敏感金黄色葡萄球菌对沙特蜂蜜的敏感性。
In vitro evaluation of methicillin-resistant and methicillin-sensitive Staphylococcus aureus susceptibility to Saudi honeys.
机构信息
Department of Microbiology, Faculty of Medicine, King Abdulaziz University, Rabigh Branch, 21589, Saudi Arabia.
Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Rabigh branch, 21589, Saudi Arabia.
出版信息
BMC Complement Altern Med. 2019 Jul 25;19(1):185. doi: 10.1186/s12906-019-2603-8.
BACKGROUND
Honey has been increasingly recognized as a potential therapeutic agent for treatment of wound infections. There is an urgent need for assessment and evaluation of the antibacterial properties against wound pathogens of honeys that have not yet been tested.
METHODS
Ten Saudi honeys collected from different geographical locations were screened initially for their antibacterial potential against methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) by the agar well diffusion method. Manuka honey (UMF-12) was used for comparison. Of the tested honeys, the honey that exhibited the greatest antibacterial activity in the agar well diffusion assay was further evaluated for its minimum inhibitory concentration (MIC) against ten MRSA clinical isolates and three American Type Culture Collection (ATCC) reference strains by the microbroth dilution method.
RESULTS
Locally produced honeys exhibited variable antibacterial activity against the tested isolates in the agar well diffusion assay. They were unable to exhibit antibacterial activity against MSSA and MRSA at 25% dilutions (w/v) in catalase solution. However, Sumra and Talha honeys showed a zone of inhibition at 50% dilutions (w/v) in catalase solution. This finding means that both honeys possess weak non-peroxide-based antibacterial activity. Moreover, Sumra honey showed a larger inhibition zone at 50 and 25% dilutions (w/v) in distilled water than Manuka honey against both MSSA and MRSA. This result demonstrates that Sumra honey has more hydrogen peroxide-related antibacterial activity or total antibacterial activity than Manuka honey. In addition, MIC results obtained through a microbroth dilution assay showed that Sumra honey inhibited the growth of all MRSA clinical isolates (n = 10) and reference strains [MRSA (ATCC 43300) and MSSA (ATCC 29213)] at lower concentrations (12.0% v/v) than those required for Manuka honey-mediated inhibition (14.0% v/v). This result means that Sumra honey has more peroxide or synergistic antibacterial activity than Manuka honey. An equivalent MIC (15.0% v/v) was observed for E. coli (ATCC 25922) between Manuka honey and Sumra honey.
CONCLUSIONS
Sumra honey may be used as an alternative therapeutic agent for infected wounds and burns, where additional hydrogen peroxide-related antibacterial activity is needed. In the future, the physiochemical characteristics of Sumra honey may be evaluated and standardized.
背景
蜂蜜作为一种潜在的治疗伤口感染的药物，其作用已得到越来越多的认可。因此，迫切需要评估和评价尚未经过测试的蜂蜜对伤口病原体的抗菌特性。
方法
采用琼脂扩散法初步筛选了 10 种来自沙特不同地理位置的蜂蜜，以评估其对耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感金黄色葡萄球菌（MSSA）的抗菌潜力。同时使用麦卢卡蜂蜜（UMF-12）作为对照。在琼脂扩散试验中，表现出最强抗菌活性的蜂蜜，通过微量肉汤稀释法进一步评估其对 10 株 MRSA 临床分离株和 3 株美国典型培养物保藏中心（ATCC）参考菌株的最低抑菌浓度（MIC）。
结果
本地生产的蜂蜜对琼脂扩散试验中的测试分离株表现出不同的抗菌活性。在 25%（w/v）过氧化氢溶液中，它们不能对 MSSA 和 MRSA 表现出抗菌活性。然而，Sumra 和 Talha 蜂蜜在 50%（w/v）过氧化氢溶液中显示出抑菌圈。这意味着这两种蜂蜜都具有较弱的非过氧化物基础抗菌活性。此外，Sumra 蜂蜜在 50%和 25%（w/v）蒸馏水稀释度下对 MSSA 和 MRSA 的抑菌圈大于麦卢卡蜂蜜。这一结果表明，Sumra 蜂蜜的过氧化物相关抗菌活性或总抗菌活性比麦卢卡蜂蜜更强。此外，通过微量肉汤稀释法获得的 MIC 结果表明，Sumra 蜂蜜在较低浓度（12.0% v/v）下抑制了所有 10 株 MRSA 临床分离株（n=10）和参考菌株[MRSA（ATCC 43300）和 MSSA（ATCC 29213）]的生长，而麦卢卡蜂蜜介导的抑制所需浓度（14.0% v/v）更高。这一结果表明，Sumra 蜂蜜的过氧化物或协同抗菌活性比麦卢卡蜂蜜更强。麦卢卡蜂蜜和 Sumra 蜂蜜对大肠杆菌（ATCC 25922）的 MIC 等效浓度（15.0% v/v）。
结论
Sumra 蜂蜜可作为治疗感染性伤口和烧伤的替代治疗药物，尤其适用于需要额外过氧化物相关抗菌活性的情况。未来，可能会对 Sumra 蜂蜜的理化特性进行评估和标准化。
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