Shenoy Vishnu Prasad, Ballal Mamatha, Shivananda Pg, Bairy Indira
Department of Microbiology, Kasturba Medical College, Manipal University, Manipal, India.
J Glob Infect Dis. 2012 Apr;4(2):102-5. doi: 10.4103/0974-777X.96770.
As natural products garner attention in the medical field due to emergence of antibiotic resistant strains of bacteria, honey is valued for its antibacterial activity.
Fifty strains of Pseudomonas aeruginosa isolated from infected wounds were evaluated for their antibacterial action using honey in comparison with different antibiotics and Dettol.
All the strains were found to be sensitive to honey at a minimum inhibitory concentration of 20% in comparison with Dettol at 10% using agar dilution method. In the second step, the time kill assay was performed on five isolates of P. aeruginosa to demonstrate the bactericidal activity of honey at different dilutions of honey ranging from 20% to 100% at regular time intervals. All the isolates of P. aeruginosa tested were killed in 12-24 h depending on the dilutions of the honey tested. Thus, honey could prevent the growth of P. aeruginosa even if it was diluted by deionized water by fivefolds in vitro. Honey had almost uniform bactericidal activity against P. aeruginosa irrespective of their susceptibility to different classes of antibiotics.
Honey which is a natural, non-toxic, and an inexpensive product has activity against the P. aeruginosa isolated from infected wounds may make it an alternative topical choice in the treatment of wound infections.
由于抗生素耐药性细菌菌株的出现，天然产物在医学领域受到关注，蜂蜜因其抗菌活性而受到重视。
使用蜂蜜对从感染伤口分离出的50株铜绿假单胞菌进行抗菌作用评估，并与不同抗生素和滴露进行比较。
采用琼脂稀释法，发现所有菌株对蜂蜜的最低抑菌浓度为20%，而滴露为10%。第二步，对5株铜绿假单胞菌进行时间杀菌试验，以证明在不同稀释度（20%至100%）的蜂蜜中，在定期时间间隔下蜂蜜的杀菌活性。根据测试蜂蜜的稀释度，所有测试的铜绿假单胞菌分离株在12至24小时内被杀死。因此，即使在体外将蜂蜜用去离子水稀释五倍，蜂蜜仍能阻止铜绿假单胞菌的生长。无论铜绿假单胞菌对不同类抗生素的敏感性如何，蜂蜜对其几乎具有一致的杀菌活性。
蜂蜜作为一种天然、无毒且廉价的产品，对从感染伤口分离出的铜绿假单胞菌具有活性，这可能使其成为治疗伤口感染的一种替代局部用药选择。
