• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Identification of critical residues in G(alpha)13 for stimulation of p115RhoGEF activity and the structure of the G(alpha)13-p115RhoGEF regulator of G protein signaling homology (RH) domain complex.鉴定 G(alpha)13 中对 p115RhoGEF 活性的刺激至关重要的残基，以及 G(alpha)13-p115RhoGEF 调节 G 蛋白信号转导同源（RH）结构域复合物的结构。
J Biol Chem. 2011 Jun 10;286(23):20625-36. doi: 10.1074/jbc.M110.201392. Epub 2011 Apr 20.
2
Activation of p115-RhoGEF requires direct association of Gα13 and the Dbl homology domain.p115-RhoGEF 的激活需要 Gα13 和 Dbl 同源结构域的直接结合。
J Biol Chem. 2012 Jul 20;287(30):25490-500. doi: 10.1074/jbc.M111.333716. Epub 2012 Jun 1.
3
Structure of the p115RhoGEF rgRGS domain-Galpha13/i1 chimera complex suggests convergent evolution of a GTPase activator.p115RhoGEF rgRGS结构域与Gα13/i1嵌合体复合物的结构表明GTP酶激活剂的趋同进化。
Nat Struct Mol Biol. 2005 Feb;12(2):191-7. doi: 10.1038/nsmb888. Epub 2005 Jan 16.
4
Regulated localization is sufficient for hormonal control of regulator of G protein signaling homology Rho guanine nucleotide exchange factors (RH-RhoGEFs).调节性定位足以实现对G蛋白信号同源性Rho鸟嘌呤核苷酸交换因子（RH-RhoGEFs）调节剂的激素控制。
J Biol Chem. 2014 Jul 11;289(28):19737-46. doi: 10.1074/jbc.M114.564930. Epub 2014 May 22.
5
Mapping the Galpha13 binding interface of the rgRGS domain of p115RhoGEF.绘制p115RhoGEF的rgRGS结构域的Gα13结合界面。
J Biol Chem. 2003 Mar 14;278(11):9912-9. doi: 10.1074/jbc.M212695200. Epub 2003 Jan 13.
6
Modification of p115RhoGEF Ser(330) regulates its RhoGEF activity.p115RhoGEF Ser(330)的修饰调节其 RhoGEF 活性。
Cell Signal. 2013 Nov;25(11):2085-92. doi: 10.1016/j.cellsig.2013.06.012. Epub 2013 Jun 29.
7
Functional consequences of G alpha 13 mutations that disrupt interaction with p115RhoGEF.破坏与p115RhoGEF相互作用的Gα13突变的功能后果。
Oncogene. 2005 Mar 24;24(13):2155-65. doi: 10.1038/sj.onc.1208414.
8
Activation of leukemia-associated RhoGEF by Galpha13 with significant conformational rearrangements in the interface.Gα13激活白血病相关RhoGEF，界面处发生显著构象重排。
J Biol Chem. 2009 Feb 20;284(8):5000-9. doi: 10.1074/jbc.M804073200. Epub 2008 Dec 12.
9
Chimeric G alpha i2/G alpha 13 proteins reveal the structural requirements for the binding and activation of the RGS-like (RGL)-containing Rho guanine nucleotide exchange factors (GEFs) by G alpha 13.嵌合型Gαi2/Gα13蛋白揭示了Gα13对含RGS样（RGL）的Rho鸟嘌呤核苷酸交换因子（GEF）的结合和激活的结构要求。
J Biol Chem. 2004 Dec 24;279(52):54283-90. doi: 10.1074/jbc.M410594200. Epub 2004 Oct 14.
10
Modular architecture and novel protein-protein interactions regulating the RGS-containing Rho guanine nucleotide exchange factors.调节含RGS的Rho鸟嘌呤核苷酸交换因子的模块化结构和新型蛋白质-蛋白质相互作用。
Methods Enzymol. 2004;390:259-85. doi: 10.1016/S0076-6879(04)90017-1.

引用本文的文献

1
Receptor-independent regulation of Gα13 by alpha-1-antitrypsin C-terminal peptides.α1抗胰蛋白酶C末端肽对Gα13的非受体依赖性调节
J Biol Chem. 2025 Feb;301(2):108136. doi: 10.1016/j.jbc.2024.108136. Epub 2024 Dec 25.
2
Interaction kinetics between p115-RhoGEF and Gα are determined by unique molecular interactions affecting agonist sensitivity.p115-RhoGEF 与 Gα 之间的相互作用动力学受影响激动剂敏感性的独特分子相互作用决定。
Commun Biol. 2022 Nov 24;5(1):1287. doi: 10.1038/s42003-022-04224-9.
3
A Gα12-specific Binding Domain in AKAP-Lbc and p114RhoGEF.A激酶锚定蛋白Lbc和p114Rho鸟嘌呤核苷酸交换因子中的Gα12特异性结合结构域
J Mol Signal. 2016 Sep 9;11:3. doi: 10.5334/1750-2187-11-3.
4
Gastrin-stimulated Gα13 Activation of Rgnef Protein (ArhGEF28) in DLD-1 Colon Carcinoma Cells.胃泌素刺激下DLD-1结肠癌细胞中Rgnef蛋白（ArhGEF28）的Gα13激活
J Biol Chem. 2015 Jun 12;290(24):15197-209. doi: 10.1074/jbc.M114.628164. Epub 2015 Apr 28.
5
Phosphorylation-mediated regulation of GEFs for RhoA.磷酸化介导的 RhoA 鸟苷酸交换因子（GEFs）调节。
Cell Adh Migr. 2014;8(1):11-8. doi: 10.4161/cam.28058. Epub 2013 Jan 1.
6
Gα12 structural determinants of Hsp90 interaction are necessary for serum response element-mediated transcriptional activation.Gα12 结构决定簇对于热休克蛋白 90 相互作用是必需的，这对于血清反应元件介导的转录激活是必要的。
Mol Pharmacol. 2014 Apr;85(4):586-97. doi: 10.1124/mol.113.088443. Epub 2014 Jan 16.
7
Modification of p115RhoGEF Ser(330) regulates its RhoGEF activity.p115RhoGEF Ser(330)的修饰调节其 RhoGEF 活性。
Cell Signal. 2013 Nov;25(11):2085-92. doi: 10.1016/j.cellsig.2013.06.012. Epub 2013 Jun 29.
8
Determinants at the N- and C-termini of Gα12 required for activation of Rho-mediated signaling.Gα12激活Rho介导信号传导所需的N端和C端决定因素。
J Mol Signal. 2013 Mar 25;8(1):3. doi: 10.1186/1750-2187-8-3.
9
The zinc-binding region of IL-2 inducible T cell kinase (Itk) is required for interaction with Gα13 and activation of serum response factor.白细胞介素 2 诱导的 T 细胞激酶（Itk）的锌结合区域是与 Gα13 相互作用和激活血清反应因子所必需的。
Int J Biochem Cell Biol. 2013 Jun;45(6):1074-82. doi: 10.1016/j.biocel.2013.02.011. Epub 2013 Feb 27.
10
Signal activation and inactivation by the Gα helical domain: a long-neglected partner in G protein signaling.Gα 螺旋域对信号的激活和失活：G 蛋白信号转导中长期被忽视的伙伴。
Sci Signal. 2012 May 29;5(226):re2. doi: 10.1126/scisignal.2003013.

本文引用的文献

1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Modulation of a GEF switch: autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF.调节鸟苷酸交换因子开关：p115-RhoGEF 固有鸟苷酸交换活性的自动抑制。
Protein Sci. 2011 Jan;20(1):107-17. doi: 10.1002/pro.542.
3
Kinetic scaffolding mediated by a phospholipase C-beta and Gq signaling complex.由磷酯酶 C-β和 Gq 信号复合物介导的动力学支架。
Science. 2010 Nov 12;330(6006):974-80. doi: 10.1126/science.1193438. Epub 2010 Oct 21.
4
Structure and function of regulator of G protein signaling homology domains.G 蛋白信号转导调节因子同源结构域的结构与功能
Prog Mol Biol Transl Sci. 2009;86:75-113. doi: 10.1016/S1877-1173(09)86004-3. Epub 2009 Oct 7.
5
On the mechanism of autoinhibition of the RhoA-specific nucleotide exchange factor PDZRhoGEF.关于RhoA特异性核苷酸交换因子PDZRhoGEF的自抑制机制
BMC Struct Biol. 2009 May 21;9:36. doi: 10.1186/1472-6807-9-36.
6
Galpha12/13 regulate epiboly by inhibiting E-cadherin activity and modulating the actin cytoskeleton.Gα12/13 通过抑制 E-钙黏蛋白活性和调节肌动蛋白细胞骨架来调控外包。
J Cell Biol. 2009 Mar 23;184(6):909-21. doi: 10.1083/jcb.200805148.
7
Recognition of the activated states of Galpha13 by the rgRGS domain of PDZRhoGEF.通过PDZRhoGEF的rgRGS结构域识别Gα13的激活状态。
Structure. 2008 Oct 8;16(10):1532-43. doi: 10.1016/j.str.2008.07.009.
8
Structure of Galphaq-p63RhoGEF-RhoA complex reveals a pathway for the activation of RhoA by GPCRs.Gαq-p63RhoGEF-RhoA复合物的结构揭示了G蛋白偶联受体激活RhoA的一条途径。
Science. 2007 Dec 21;318(5858):1923-7. doi: 10.1126/science.1147554.
9
Distinct regions of Galpha13 participate in its regulatory interactions with RGS homology domain-containing RhoGEFs.Gα13的不同区域参与其与含RGS同源结构域的Rho鸟苷酸交换因子的调控相互作用。
Cell Signal. 2007 Aug;19(8):1681-9. doi: 10.1016/j.cellsig.2007.03.004. Epub 2007 Mar 15.
10
Structural basis of function in heterotrimeric G proteins.异源三聚体G蛋白功能的结构基础
Q Rev Biophys. 2006 May;39(2):117-66. doi: 10.1017/S0033583506004306. Epub 2006 Aug 21.

鉴定 G(alpha)13 中对 p115RhoGEF 活性的刺激至关重要的残基，以及 G(alpha)13-p115RhoGEF 调节 G 蛋白信号转导同源（RH）结构域复合物的结构。

Identification of critical residues in G(alpha)13 for stimulation of p115RhoGEF activity and the structure of the G(alpha)13-p115RhoGEF regulator of G protein signaling homology (RH) domain complex.

机构信息

Department of Pharmacology, College of Medicine, University of Illinois, Chicago, Illinois 60612, USA.

出版信息

J Biol Chem. 2011 Jun 10;286(23):20625-36. doi: 10.1074/jbc.M110.201392. Epub 2011 Apr 20.

DOI:10.1074/jbc.M110.201392
PMID:21507947
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3121507/
Abstract

RH-RhoGEFs are a family of guanine nucleotide exchange factors that contain a regulator of G protein signaling homology (RH) domain. The heterotrimeric G protein Gα(13) stimulates the guanine nucleotide exchange factor (GEF) activity of RH-RhoGEFs, leading to activation of RhoA. The mechanism by which Gα(13) stimulates the GEF activity of RH-RhoGEFs, such as p115RhoGEF, has not yet been fully elucidated. Here, specific residues in Gα(13) that mediate activation of p115RhoGEF are identified. Mutation of these residues significantly impairs binding of Gα(13) to p115RhoGEF as well as stimulation of GEF activity. These data suggest that the exchange activity of p115RhoGEF is stimulated allosterically by Gα(13) and not through its interaction with a secondary binding site. A crystal structure of Gα(13) bound to the RH domain of p115RhoGEF is also presented, which differs from a previously crystallized complex with a Gα(13)-Gα(i1) chimera. Taken together, these data provide new insight into the mechanism by which p115RhoGEF is activated by Gα(13).

摘要

RH-RhoGEFs 是一类鸟苷酸交换因子，包含调节 G 蛋白信号同源（RH）结构域。异三聚体 G 蛋白 Gα(13)刺激 RH-RhoGEFs 的鸟苷酸交换因子（GEF）活性，导致 RhoA 的激活。但是，Gα(13) 如何刺激 RH-RhoGEFs（如 p115RhoGEF）的 GEF 活性尚未完全阐明。在这里，确定了介导 p115RhoGEF 激活的 Gα(13)中的特定残基。这些残基的突变显著损害了 Gα(13)与 p115RhoGEF 的结合以及 GEF 活性的刺激。这些数据表明，p115RhoGEF 的交换活性是通过 Gα(13)的变构刺激，而不是通过与辅助结合位点的相互作用。还呈现了与 p115RhoGEF 的 RH 结构域结合的 Gα(13)的晶体结构，该结构与先前与 Gα(13)-Gα(i1)嵌合体的结晶复合物不同。这些数据共同为 p115RhoGEF 被 Gα(13)激活的机制提供了新的见解。