College of Life Science Zhejiang University, 310058, Hangzhou, Zhejiang Province, China.
Water Sci Technol. 2011;63(7):1531-8. doi: 10.2166/wst.2011.328.
Pseudomonas sp. strain DY1 was newly isolated from soil with rotten wood and identified as a member of the genus Pseudomonas based on 16S rDNA and biochemical tests. Acid Black 172, a water soluble Cr-complex dye, was then selected as a model dye to investigate the decolorisation ability of the strain. It was observed that the growth of the strain was not inhibited by high dose of metal ions (10 mM), and efficient decolorisation was still observed when high concentrations of Fe(2+), Fe(3+) and Ca(2+) existed. The optimal decolorising conditions obtained from Taguchi design were as follows: temperature 37˚C, pH 7.0, Fe(3+) and proline concentrations of 7 mM and 3.0 g/L, respectively. Under the optimal conditions, 94.5% of Acid Black 172 (100 mg/L) could be decolorised by the strain in 24 h. The kinetics of the decolorisation best fitted the first order kinetic model (R(2)=0.981). Besides, the phytotoxicity study demonstrated a good detoxification by the strain. This work has a certain practical value in microbial decolorisation of textile wastewater.
DY1 菌株是从腐烂木材土壤中分离出来的一株新的假单胞菌，根据 16S rDNA 和生化试验鉴定为假单胞菌属的一个成员。然后选择水溶性 Cr 络合染料酸性黑 172 作为模型染料，研究该菌株的脱色能力。结果表明，该菌株的生长不受高剂量金属离子（10 mM）的抑制，当存在高浓度的 Fe(2+)、Fe(3+)和 Ca(2+)时，仍能有效脱色。通过田口设计得到的最佳脱色条件如下：温度 37°C，pH 值 7.0，Fe(3+)和脯氨酸的浓度分别为 7 mM 和 3.0 g/L。在最佳条件下，该菌株可在 24 h 内将 100 mg/L 的酸性黑 172 脱色 94.5%。脱色动力学最佳拟合一级动力学模型（R(2)=0.981）。此外，毒性研究表明该菌株具有良好的解毒作用。这项工作在微生物处理纺织废水的脱色方面具有一定的实用价值。
