Laboratory of Enzyme Engineering and Microbiology, National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia.
Unité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia.
J Appl Microbiol. 2019 Jun;126(6):1761-1771. doi: 10.1111/jam.14262. Epub 2019 Apr 29.
The effect of heavy metals presence on the decolourization of Reactive Black 5 by Pseudomonas aeruginosa was evaluated.
In the current study, a newly isolated strain identified as P. aeruginosa strain Gb 30 was selected for its ability to remove high concentration of Reactive Black 5 and resistance to several heavy metals (Cu >Zn >Cd >Cr ). Strain Gb30 was used to assess the effect of heavy metals presence on RB5 decolourization. The strain growth exhibited different responses at a fixed concentration of EC (10 h) for each heavy metal. The addition of Zn and Cd had no effect on decolourization yield after 24 h of incubation, whereas Cr and Cu ions reduced decolourization up to 17%. In order to understand the relationship between heavy metals contamination and decolourization, experimental data relating the initial decolourization rate of RB5 to the concentrations of single and associated heavy metals were fitted to three different inhibition kinetic models.
In this study, we showed that P. aeruginosa strain Gb30 could be used for dye removal even at high concentrations of heavy metals. The developed models could provide basic information that may help for the best management of the bacteria-mediated decolourization process at the industrial scale.
This study opens new directions for the management of textile industry wastewaters containing dyes and heavy metals using bioaugmentation by P. aeruginosa strain Gb30.
评估重金属存在对铜绿假单胞菌对活性黑 5 脱色的影响。
在本研究中，选择了一株新分离的铜绿假单胞菌菌株 Gb 30，因其能够去除高浓度的活性黑 5 并对几种重金属（Cu>Zn>Cd>Cr）具有抗性。使用菌株 Gb30 来评估重金属存在对 RB5 脱色的影响。在每种重金属的固定 EC（10 h）浓度下，菌株生长表现出不同的反应。在 24 h 的孵育后，Zn 和 Cd 的添加对脱色产率没有影响，而 Cr 和 Cu 离子将脱色率降低了 17%。为了了解重金属污染与脱色之间的关系，将 RB5 的初始脱色率与单一和共存重金属浓度之间的实验数据拟合到三种不同的抑制动力学模型中。
在这项研究中，我们表明铜绿假单胞菌菌株 Gb30 即使在高浓度重金属存在的情况下也可用于染料去除。所开发的模型可以提供基本信息，这可能有助于在工业规模上对细菌介导的脱色过程进行最佳管理。
本研究为使用铜绿假单胞菌菌株 Gb30 通过生物增强来管理含有染料和重金属的纺织工业废水开辟了新的方向。
