Arkema J M, Broekhuis-Fluitsma D M, de Laat P A, Hoefsmit E C
Dept. of Cell Biology, Free University, Amsterdam, The Netherlands.
Immunobiology. 1990 Nov;181(4-5):335-44. doi: 10.1016/S0171-2985(11)80502-4.
The localization of histocompatibility antigens of the HLA-D locus in dendritic cells (DC) and monocytes (Mo) isolated from human peripheral blood was investigated. Functionally DC were characterized by their capacity to act as strong stimulators in an allogeneic mixed leucocyte reaction. In cytospins, DC were differentiated from Mo by dendritic morphology, strong HLA class II and moderate RFD1 expression on the plasma membrane and acid phosphatase activity in a juxtanuclear spot. Ultrathin cryosections showed that DC had a heavily labelled plasma membrane for HLA-D. In addition, these antigens were found in intracellular vesicles predominantly located in the juxtanuclear zone. This pattern of labelling was not seen in Mo. Obviously, DC concentrate intracellular class II antigens in the same area as lysosomal activity. These results may indicate that this juxtanuclear area is a center of antigen processing in DC.
研究了从人外周血分离的树突状细胞（DC）和单核细胞（Mo）中HLA - D位点组织相容性抗原的定位。在功能上，DC的特征在于其在同种异体混合白细胞反应中作为强刺激剂的能力。在细胞涂片上，DC通过树突形态、质膜上强HLA II类和中度RFD1表达以及近核点处的酸性磷酸酶活性与Mo区分开来。超薄冷冻切片显示DC的HLA - D质膜标记强烈。此外，这些抗原存在于主要位于近核区的细胞内囊泡中。Mo中未观察到这种标记模式。显然，DC在与溶酶体活性相同的区域浓缩细胞内II类抗原。这些结果可能表明这个近核区域是DC中抗原加工的中心。
