Knight S C, Fryer P, Griffiths S, Harding B
Immunology. 1987 May;61(1):21-7.
Histocompatibility antigens of the HLA D locus on the surface of human dendritic cells (DC) were visualized in the electron microscope using immunogold labelling. DC from peripheral blood expressed DR that was frequently concentrated at junctions between aggregating DC and lymphocytes or DC and macrophages. Labelling with an antibody to DQ was more diffuse and was not concentrated at points of cell-cell contact. The D locus antibody RFD1 labelled DC in distinct patches that were sometimes located at points of cell contact. Upon labelling DC with antibody to DR and incubating the cells at 37 degrees, some label remained on the cell surface but some was found in deep channels which appeared to be formed between veils at the surface of the cell and became internalized in membrane-bound structures. Under the same conditions, gold bound to DQ molecules remained on the surface of DC. Gold labelling RFD1 also remained mainly on the cell surface but there was occasionally internalization of patches into the cells through depressions in the cell membrane. The changes in distribution of the label on warming the cells suggests that materials bound to different D locus products may be 'processed' differently.
利用免疫金标记技术，在电子显微镜下观察了人树突状细胞（DC）表面HLA D位点的组织相容性抗原。外周血来源的DC表达DR，其常集中于聚集的DC与淋巴细胞或DC与巨噬细胞之间的连接处。用抗DQ抗体标记时，标记更为弥散，并不集中于细胞间接触点。D位点抗体RFD1以不同的斑块形式标记DC，这些斑块有时位于细胞接触点。用抗DR抗体标记DC并在37℃孵育细胞后，一些标记物仍留在细胞表面，但有些则出现在深层通道中，这些通道似乎是在细胞表面的面纱之间形成的，并被内化到膜结合结构中。在相同条件下，与DQ分子结合的金仍留在DC表面。标记RFD1的金也主要留在细胞表面，但偶尔会有斑块通过细胞膜凹陷内化到细胞中。细胞升温时标记物分布的变化表明，与不同D位点产物结合的物质可能有不同的“处理”方式。
