Wound Healing Research Group, Department of Surgery, Division of Plastic and Reconstructive Surgery, University of Alberta, Edmonton, Alberta, Canada.
Wound Repair Regen. 2011 May-Jun;19(3):368-78. doi: 10.1111/j.1524-475X.2011.00677.x. Epub 2011 Apr 21.
Small leucine-rich proteoglycans (SLRPs) are extracellular matrix molecules that regulate collagen fibrillogenesis and inhibit transforming growth factor-β activity; thus, they may play a critical role in wound healing and scar formation. Hypertrophic scarring is a dermal form of fibroproliferative disorders, which occurs in over 70% of burn patients and leads to disfigurement and limitations in function. By understanding the cellular and molecular mechanisms that lead to scarring after injury, new clinical therapeutic approaches can by developed to minimize abnormal scar formation in hypertrophic scarring and other fibroproliferative disorders. To study the expression and localization of SLRPs with connective tissue cells in tissue immunohistochemistry, immunofluorescence staining, immunoblotting, and reverse-transcription polymerase chain reaction were used in normal skin and hypertrophic scar (HTS). In normal skin, there was more decorin and fibromodulin accumulation in the superficial layers than in the deeper dermal layers. The levels of decorin and fibromodulin were significantly lower in HTS, whereas biglycan was increased when compared with normal skin. There was an increased expression of biglycan, fibromodulin, and lumican in the basement membrane and around basal epithelial cells. In contrast, these proteoglycans were absent or weakly expressed in HTS. The findings suggest that down-regulation of SLRPs after wound healing in deep injuries to the skin plays an important role in the development of fibrosis and HTS.
小富含亮氨酸的蛋白聚糖（SLRPs）是细胞外基质分子，可调节胶原纤维原纤维的形成并抑制转化生长因子-β的活性；因此，它们可能在伤口愈合和瘢痕形成中起关键作用。增生性瘢痕是一种皮肤形式的纤维增生性疾病，发生在超过 70%的烧伤患者中，并导致毁容和功能受限。通过了解导致受伤后瘢痕形成的细胞和分子机制，可以开发新的临床治疗方法，以最大限度地减少增生性瘢痕和其他纤维增生性疾病中异常瘢痕的形成。为了在组织免疫组织化学、免疫荧光染色、免疫印迹和逆转录聚合酶链反应中研究与结缔组织细胞相关的 SLRPs 的表达和定位，使用正常皮肤和增生性瘢痕（HTS）进行了研究。在正常皮肤中，浅层的聚集素和纤维调节素积累比深层真皮层多。HTS 中的聚集素和纤维调节素水平明显降低，而 biglycan 与正常皮肤相比则增加。在基底膜和基底上皮细胞周围，biglycan、fibromodulin 和 lumican 的表达增加。相比之下，这些蛋白聚糖在 HTS 中缺失或表达较弱。这些发现表明，皮肤深部伤口愈合后 SLRPs 的下调在纤维化和 HTS 的发展中起重要作用。
