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过表达纤调蛋白的成纤维细胞可增加伤口收缩、改善瘢痕质量并促进血管生成：一项体内研究。

Fibromodulin-overexpressing fibroblast cells increase wound contraction, improve scar quality and enhance angiogenesis: an in-vivo study.

作者信息

Abdollahzadeh Negar, Vatanchian Mehran, Oroojalian Fatemeh, Enderami Seyed Ehsan, Amani Amir, Salarinia Reza

机构信息

Department of Advanced Technologies, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran.

Department of Anatomical Sciences and Pathology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran.

出版信息

BMC Biotechnol. 2025 May 19;25(1):40. doi: 10.1186/s12896-025-00975-5.

DOI:10.1186/s12896-025-00975-5
PMID:40389960
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12090437/
Abstract

INTRODUCTION

Fibromodulin, a small leucine rich proteoglycan has been suggested to have prominent role in wound healing. On the other hand, fibroblast cells, due to their ability to secrete growth factors and control inflammation in the wound area, have been proposed as effective approaches in cell therapy for wounds. In the current study we attempted to improve treatment results using a combination of fibroblast and fibromodulin features.

METHOD

Fibroblast cells were isolated from the skin and transfected with a vector carrying the fibromodulin gene. Following the assessment of fibromodulin protein production, the effect of transfected fibroblast cells was studied in an animal wound model.

RESULTS

Flow cytometry analysis showed high expression of the CD90 marker (97.2%) and very low expression of the CD34 marker (0.47%). Additionally, enzyme-linked immunosorbent assay (ELISA) findings confirmed high expression of the fibromodulin gene in the transfected fibroblast cells. In vivo studies demonstrated that the animals treated with fibroblast cells transfected with fibromodulin (V + G+) exhibited significantly improved wound contraction on day 7 (i.e., contraction percentage: 21.79 ± 9.96%, compared with 7.23 ± 2.30% in the PBS-treated group). Histopathological studies also indicated improvements in angiogenesis score and collagen density score in the animals treated with the V + G + group.

CONCLUSION

The results of this study showed that fibroblast cells expressing the fibromodulin gene improve wound contraction and some histological parameters in the deep wound model of the rat.

摘要

引言

纤维调节素是一种富含亮氨酸的小分子蛋白聚糖，已被认为在伤口愈合中发挥重要作用。另一方面，成纤维细胞由于能够分泌生长因子并控制伤口区域的炎症，已被提议作为伤口细胞治疗的有效方法。在本研究中，我们试图结合成纤维细胞和纤维调节素的特性来改善治疗效果。

方法

从皮肤中分离出成纤维细胞，并用携带纤维调节素基因的载体进行转染。在评估纤维调节素蛋白产生后，在动物伤口模型中研究转染的成纤维细胞的作用。

结果

流式细胞术分析显示CD90标记物高表达（97.2%），CD34标记物低表达（0.47%）。此外，酶联免疫吸附测定（ELISA）结果证实转染的成纤维细胞中纤维调节素基因高表达。体内研究表明，用纤维调节素转染的成纤维细胞（V+G+）治疗的动物在第7天伤口收缩明显改善（即收缩百分比：21.79±9.96%，而PBS治疗组为7.23±2.30%）。组织病理学研究还表明，V+G+组治疗的动物在血管生成评分和胶原密度评分方面有所改善。

结论

本研究结果表明，表达纤维调节素基因的成纤维细胞可改善大鼠深部伤口模型中的伤口收缩和一些组织学参数。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/32ba2b22b63f/12896_2025_975_Fig12_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/604c19a19ad6/12896_2025_975_Fig5_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/32ba2b22b63f/12896_2025_975_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/caae780162aa/12896_2025_975_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/464b6789211e/12896_2025_975_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/6e36ca4f54d6/12896_2025_975_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/bd1be1d7e5d9/12896_2025_975_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/604c19a19ad6/12896_2025_975_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/db0868653daa/12896_2025_975_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/e6690d2ea8e7/12896_2025_975_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/239024d35b42/12896_2025_975_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/e2798091d902/12896_2025_975_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/c08685488452/12896_2025_975_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/827658e6171b/12896_2025_975_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4ea/12090437/32ba2b22b63f/12896_2025_975_Fig12_HTML.jpg

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