Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.
Cell Biochem Funct. 2011 Jul;29(5):378-83. doi: 10.1002/cbf.1760. Epub 2011 Apr 25.
MicroRNAs (miRNAs) are small non-coding RNAs that participate in diverse biological processes including skeletal muscle development. MiR-214 is an miRNA that is differentially expressed in porcine embryonic muscle and adult skeletal muscle, suggesting that miR-214 may be related to embryonic myogenesis. In this study, the myoblast cell line C2C12 was used for functional analysis of miR-214 in vitro. The results showed that miR-214 was expressed both in myoblasts and in myotubes and was upregulated during differentiation. After treatment with an miR-214 inhibitor and culturing in differentiation medium, myoblast differentiation was repressed, as indicated by the significant downregulation of expression of the myogenic markers myogenin and myosin heavy chain (MyHC). Interestingly, myoblast proliferation was also repressed when cells were transfected with an miR-214 inhibitor and cultured in growth medium by real-time proliferation assay and cell cycle analysis. Our results showed that miR-214 regulates both proliferation and differentiation of myoblasts depending on the conditions.
微小 RNA（miRNA）是一种小的非编码 RNA，参与多种生物学过程，包括骨骼肌发育。miR-214 是一种在猪胚胎肌肉和成年骨骼肌中差异表达的 miRNA，提示 miR-214 可能与胚胎肌发生有关。在这项研究中，使用成肌细胞系 C2C12 进行 miR-214 的体外功能分析。结果表明，miR-214 在成肌细胞和成肌管中均有表达，并在分化过程中上调。用 miR-214 抑制剂处理并在分化培养基中培养后，成肌细胞分化受到抑制，肌生成标志物肌生成素和肌球蛋白重链（MyHC）的表达显著下调。有趣的是，当细胞用 miR-214 抑制剂转染并在生长培养基中培养时，通过实时增殖测定和细胞周期分析，成肌细胞的增殖也受到抑制。我们的结果表明，miR-214 根据不同的条件调节成肌细胞的增殖和分化。
