Chen Xiaoling, Huang Zhiqing, Chen Daiwen, Yang Ting, Liu Guangmang
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, P. R. China.
Cell Biol Int. 2014 Feb;38(2):266-71. doi: 10.1002/cbin.10192. Epub 2013 Oct 30.
MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that are critically involved in roles in various aspects of skeletal myogenesis. microRNA miR-27a promotes myoblast proliferation by targeting myostatin, a critical inhibitor of skeletal muscle development, but its mode of action in myoblast differentiation remains unclear. We have found that expression of miR-27a and myostatin were upregulated and downregulated, respectively, during myoblast differentiation. Overexpression of miR-27a increased the number of myosin heavy chain (MHC)-positive cells and upregulated mRNA and protein of MyoD and myogenin. These findings indicate that miR-27a plays a role in enhancing myoblast differentiation.
微小RNA（miRNA）是一类内源性非编码RNA，在骨骼肌生成的各个方面发挥着关键作用。微小RNA miR-27a通过靶向肌肉生长抑制素促进成肌细胞增殖，肌肉生长抑制素是骨骼肌发育的关键抑制剂，但其在成肌细胞分化中的作用模式仍不清楚。我们发现，在成肌细胞分化过程中，miR-27a的表达上调，而肌肉生长抑制素的表达下调。miR-27a的过表达增加了肌球蛋白重链（MHC）阳性细胞的数量，并上调了MyoD和肌细胞生成素的mRNA及蛋白质水平。这些发现表明，miR-27a在增强成肌细胞分化中发挥作用。
